通过流式细胞术分析慢性淋巴细胞白血病（CLL）和急性淋巴细胞白血病（ALL）患者细胞的免疫表型特性关联。

Immunophenotypic properties association of CLL and ALL patient cells by flow cytometry analysis.

作者信息

Rasul Khder H, Wsoo Mohammed A, Hassan Dlshad H, Hamadamin Shler Kh, Qadr Zainab J

机构信息

Department of Biology, College of Science, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq.

Medical Analysis Department, Tishk International University, Erbil, Iraq.

出版信息

J Taibah Univ Med Sci. 2024 Sep 24;19(5):981-989. doi: 10.1016/j.jtumed.2024.09.008. eCollection 2024 Oct.

DOI:10.1016/j.jtumed.2024.09.008

PMID:39397873

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11470534/

Abstract

UNLABELLED

Chronic lymphocytic leukemia (CLL) and acute lymphoblastic leukemia (ALL) are blood cancers that affect lymphocytes and can be diagnosed by flow cytometry. Flow cytometry is a laboratory technique that analyzes cell properties, including cell surface markers such as cluster of differentiation 19 (CD19).

OBJECTIVE

The main objective of this study was to explore the correlation of the number of CD19-positive cells with other CD antigens in patients with CLL and ALL.

METHODS

After receiving ethical approval (Approval No. 5S/401), blood was collected from participants who had been diagnosed by a physician. Then the collected blood was prepared for flow cytometry analysis according to the protocol by staining with fluorescent antibodies.

RESULTS

The results of the current study showed that sex and different age groups had no statistical influence on the number of CD19-positive cells in the patients evaluated. The generated models did not reveal an association with the number of CD19-positive cells in patients with CLL and ALL. In patients with CLL, the number of cells expressing CD5, CD20, CD23, and CD200 was significantly and positively related with the number of CD19-positive cells. In patients with ALL, the number of cells expressing CD79 and CD99 was significantly and positively correlated with the number of CD19-positive cells. This comparison study also found that in patients with CLL, the number of CD19-positive cells was significantly higher than the number of cells expressing CD20, CD23, and CD200. In patents with ALL, there was a significantly higher number of CD19-positive cells than CD34-positive and CD79-positive cells.

CONCLUSION

In patients with CLL, there was a strong positive correlation between the number of CD19-positive cells and the number of cells expressing CD5, CD20, CD23, and CD200. Additionally, in patients with ALL, there was a positive correlation of CD79 and CD99 with the number of CD19-positive cells.

摘要

未标注

慢性淋巴细胞白血病（CLL）和急性淋巴细胞白血病（ALL）是影响淋巴细胞的血癌，可通过流式细胞术进行诊断。流式细胞术是一种分析细胞特性的实验室技术，包括细胞表面标志物，如分化簇19（CD19）。

目的

本研究的主要目的是探讨CLL和ALL患者中CD19阳性细胞数量与其他CD抗原的相关性。

方法

在获得伦理批准（批准号5S/401）后，从经医生诊断的参与者中采集血液。然后根据方案用荧光抗体染色，将采集的血液制备用于流式细胞术分析。

结果

本研究结果表明，性别和不同年龄组对所评估患者的CD19阳性细胞数量无统计学影响。所生成的模型未显示与CLL和ALL患者的CD19阳性细胞数量有关联。在CLL患者中，表达CD5、CD20、CD23和CD200的细胞数量与CD19阳性细胞数量呈显著正相关。在ALL患者中，表达CD79和CD99的细胞数量与CD19阳性细胞数量呈显著正相关。这项比较研究还发现，在CLL患者中，CD19阳性细胞数量显著高于表达CD20、CD23和CD200的细胞数量。在ALL患者中，CD19阳性细胞数量显著高于CD34阳性和CD79阳性细胞数量。