Fan Ronghua, Zhang Xiwen, Wu Jie, Zhang Luwei, Chen Weiyun, Yang Qiaozhi, Zheng Rui, Zhang Lifeng, Li Xin, Xu Kebin
School of Public Health, Shenyang Medical College, Shenyang 110034, People's Republic of China.
School of Basic Medicine, Shenyang Medical College, Shenyang 110034, People's Republic of China.
ACS Omega. 2024 Sep 26;9(40):41217-41224. doi: 10.1021/acsomega.4c02230. eCollection 2024 Oct 8.
The significance of the detection of phosphate ions is immense in the realms of chemistry, biology, medicine, environment, and industry. The detection of phosphate ions is currently mainly reliant on blue molybdenum colorimetry, which is accurate but requires sample pretreatment, intricate operation, and a high price tag. Consequently, it is essential to create a sensor with superior efficiency, precision, straightforward functioning, and instantaneous online detection. This study has designed and created an electrochemical modification based on an iron metal electrode for this purpose. Cyclic voltammetry was used to initially ascertain the potential (-0.57 V) necessary for constant potential electrolysis. Employing a constant potential electrolysis method, the iron oxide and its phosphate were modified onto the surface of the iron electrode to enable reaction with phosphate ions. Scanning electron microscopy and energy dispersive X-ray spectroscopy were used to characterize and analyze the morphology and elemental composition of Fe-PME, elucidating how it responds to phosphate ionation. The two-electrode system was then utilized for the evaluation of the phosphate ion response of Fe-PME at pH 4. Fe-PME's response to phosphate ions is demonstrated by the results, ranging from 10 to 0.1 M and with a slope of -52.8 mV dec. Fe-PME exhibited satisfactory results when compared to the conventional blue colorimetry of molybdenum.
磷酸根离子的检测在化学、生物学、医学、环境和工业领域具有重大意义。目前，磷酸根离子的检测主要依赖于钼蓝比色法，该方法准确但需要样品预处理、操作复杂且成本高昂。因此，开发一种高效、精确、操作简便且能实时在线检测的传感器至关重要。本研究为此设计并制作了一种基于铁金属电极的电化学修饰物。首先采用循环伏安法确定恒电位电解所需的电位（-0.57 V）。采用恒电位电解法，将氧化铁及其磷酸盐修饰在铁电极表面，使其能与磷酸根离子发生反应。利用扫描电子显微镜和能量色散X射线光谱对铁-磷修饰电极（Fe-PME）的形貌和元素组成进行表征和分析，阐明其对磷酸根离子化的响应方式。然后利用两电极系统评估Fe-PME在pH为4时对磷酸根离子的响应。结果表明，Fe-PME对磷酸根离子的响应范围为10至0.1 M，斜率为-52.8 mV/dec。与传统的钼蓝比色法相比，Fe-PME表现出令人满意的结果。
