Synthesis and evaluation of antibacterial and antibiofilm agents based on phenylamino-substituted 1,4-benzoquinones.

This work describes the synthesis and antimicrobial evaluation of 6-aminated 1,4-benzoquinones (6-AQs) against seven resistant pathogens. The 6-AQs, synthesized via a Michael addition reaction between bromoquinone and -substituted anilines, were assessed for their antimicrobial activity through both and analyses. Bromoquinone and 6-AQs with electron-withdrawing groups demonstrated activity against , with minimum inhibitory concentrations ranging from 16 to 128 μg/ml, comparable to standard antimicrobials. Two derivatives exhibited minimum inhibitory concentrations values against methicillin-resistant ranging from 64 to 128 μg/ml. These compounds demonstrated both bacteriostatic and bactericidal effects, and antibiofilm features. The 6-AQs and showed a promising antimicrobial profile, indicating their potential as new therapeutic options.