Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung 91201, Taiwan.
International Degree Program in Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Avian Dis. 2024 Sep;68(3):183-191. doi: 10.1637/aviandiseases-D-24-00032.
Fowl cholera, caused by infection, poses challenges for prevention because of its many serotypes. Bacterins are currently widely used for vaccination against fowl cholera, but protection is limited to homologous strains. Live attenuated vaccines of provide some heterologous protection, but side effects are considerable. More recently, protein-based antigens are promising subunit vaccines when their low immunogenicity has been addressed with effective adjuvants. Bacterial flagellin has been widely considered a promising adjuvant for vaccines. In this study, we tested the adjutancy of flagellin in a subunit vaccine against in a mice and chicken models. For vaccine formulation, the antigen fPlpE ( liporotein E) was combined with fFliC ( Typhimurium flagellin). The recombinant proteins of fPlpE and fFliC were successfully expressed using the system as the expected sizes of 55 kDa and 70 kDa, respectively. The fFliC elicited strong expression levels of proinflammatory cytokine (IL-1β, IL-8, and IL-6) when stimulated in native chicken peripheral blood mononuclear cells. Immunization of mice and chickens with the subunit vaccines containing fFliC accelerated the antibody response. In the challenge tests, fFliC increased vaccine protective efficacy against the heterologous strain A1 and highly virulent strain Chu01 in mice and chickens, respectively. These data indicated potential possibilities of using fFliC as an immunostimulant adjuvant in developing a subunit vaccine against fowl cholera.
禽霍乱由 感染引起，由于其多种血清型，给预防带来了挑战。菌苗目前广泛用于预防禽霍乱，但保护作用仅限于同源菌株。减毒活疫苗 可提供一定的异源保护，但副作用相当大。最近，基于蛋白质的抗原作为亚单位疫苗具有很大的应用前景，其低免疫原性已通过有效的佐剂得到解决。细菌鞭毛蛋白已被广泛认为是疫苗的一种有前途的佐剂。在这项研究中，我们在小鼠和鸡模型中测试了鞭毛蛋白在针对 的亚单位疫苗中的佐剂作用。在疫苗配方中，抗原 fPlpE（脂蛋白 E）与 fFliC（沙门氏菌鞭毛蛋白）结合。使用 系统成功表达了 fPlpE 和 fFliC 的重组蛋白，分别为预期的 55 kDa 和 70 kDa。当刺激天然鸡外周血单核细胞时，fFliC 引发了强烈的促炎细胞因子（IL-1β、IL-8 和 IL-6）的表达。用含有 fFliC 的亚单位疫苗免疫小鼠和鸡加速了抗体反应。在攻毒试验中，fFliC 分别提高了疫苗对异源菌株 A1 和高毒力菌株 Chu01 在小鼠和鸡中的保护效力。这些数据表明，fFliC 作为一种免疫刺激佐剂，有可能用于开发针对禽霍乱的亚单位疫苗。
