Centre for Animal Sciences and Veterinary Studies (CECAV), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.
Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 5000-801 Vila Real, Portugal.
Food Funct. 2024 Oct 28;15(21):10795-10810. doi: 10.1039/d4fo03217a.
The integrity of the genome is under constant threat from both endogenous and exogenous factors that induce oxidative stress and accelerate ageing. The demand for natural and organic cosmetics is rising due to the harmful effects of synthetic genotoxic agents on human health and the environment. Elderberry ( L.), a fruit rich in bioactive compounds such as polyphenols, has demonstrated significant antioxidant properties. This study aimed to evaluate elderberry extract's chemical characterization and biological activities in peripheral blood mononuclear cells exposed to streptonigrin and HO, both known for causing DNA damage. The antigenotoxic evaluation and antioxidant assays (ABTS and DPPH) were conducted to assess its biological properties. Using the Comet assay enhanced with formamidopyrimidine-DNA glycosylase (Fpg) to detect oxidized purines, we found that elderberry extract significantly reduced DNA damage. These findings suggest that elderberry has potential as a natural alternative to synthetic ingredients in cosmetics, offering protective benefits against DNA damage and contributing to anti-ageing and skin health.
基因组的完整性不断受到内源性和外源性因素的威胁，这些因素会引发氧化应激并加速衰老。由于合成遗传毒性物质对人类健康和环境的有害影响，对天然和有机化妆品的需求正在上升。接骨木（L.）是一种富含生物活性化合物的水果，如多酚，具有显著的抗氧化特性。本研究旨在评估接骨木提取物在暴露于streptonigrin 和 HO 的外周血单核细胞中的化学特征和生物活性，这两种物质都已知会导致 DNA 损伤。通过抗原毒性评估和抗氧化测定（ABTS 和 DPPH）来评估其生物特性。使用经 formamidopyrimidine-DNA glycosylase (Fpg) 增强的彗星试验来检测氧化嘌呤，我们发现接骨木提取物可显著降低 DNA 损伤。这些发现表明，接骨木提取物具有作为化妆品中合成成分替代品的潜力，提供了对 DNA 损伤的保护益处，并有助于抗衰老和皮肤健康。
