Qian Hengwei, Xiao Zhiliang, Cheng Lirui, Geng Ruimei, Ma Yan, Bi Yanxiao, Liang Wenxing, Yang Aiguo
Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.
College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
Plant Cell Environ. 2025 Feb;48(2):1021-1036. doi: 10.1111/pce.15200. Epub 2024 Oct 13.
Fusarium oxysporum, an important soilborne fungal pathogen that causes serious Fusarium wilt disease, secretes diverse effectors during the infection. In this study, we identified a novel secreted cysteine-rich protein, FolSCP1, which contains unknown protein functional domain. Here, we characterized FolSCP1 as a secreted virulence factor that promotes the pathogen infection of host plants by inhibiting diverse plant defence responses. FolSCP1 interacted with the pathogenesis-related 5 (PR-5) protein SlPR5, a positive regulator of tomato plant immunity against multiple tomato pathogens, and effectively attenuated the antifungal activity of the tomato PR-5 protein. FoSCP1, a homologue of FolSCP1, was secreted by a F. oxysporum isolate from infected tobacco and targeted the tobacco PR-5 protein NtPR5 to suppress plant defence for further infection. In summary, our study revealed a fungal virulence strategy in which F. oxysporum secrete effectors that interfere with plant immunity by binding to the PR-5 protein of the host plant and inhibiting its biological activity, thereby promoting fungal infection.
尖孢镰刀菌是一种重要的土传真菌病原体，可引发严重的镰刀菌枯萎病，在感染过程中会分泌多种效应蛋白。在本研究中，我们鉴定出一种新型的富含半胱氨酸的分泌蛋白FolSCP1，其含有未知的蛋白质功能结构域。在此，我们将FolSCP1表征为一种分泌型毒力因子，它通过抑制多种植物防御反应来促进病原体对宿主植物的感染。FolSCP1与病程相关蛋白5（PR-5）SlPR5相互作用，SlPR5是番茄植株对多种番茄病原体免疫的正向调节因子，FolSCP1可有效减弱番茄PR-5蛋白的抗真菌活性。FoSCP1是FolSCP1的同源物，由一株从受感染烟草中分离出的尖孢镰刀菌分泌，靶向烟草PR-5蛋白NtPR5以抑制植物防御从而进一步感染。总之，我们的研究揭示了一种真菌毒力策略，即尖孢镰刀菌分泌效应蛋白，通过与宿主植物的PR-5蛋白结合并抑制其生物活性来干扰植物免疫，从而促进真菌感染。
