Xu Zhenchuang, Wang Chenyang, He Shengyuan, Wu Jian, Zhao Yanchuan
Key Laboratory of Fluorine and Nitrogen Chemistry and Advanced Materials and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Ling-Ling Road, Shanghai, 200032, China.
Instrumental Analysis Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Ling-Ling Road, Shanghai, 200032, China.
Angew Chem Int Ed Engl. 2025 Jan 27;64(5):e202417112. doi: 10.1002/anie.202417112. Epub 2024 Nov 9.
Real-time monitoring of molecular transformations is crucial for advancements in biotechnology. In this study, we introduce a novel self-assembling F-labeled nuclear magnetic resonance (NMR) probe that disassembles upon interaction with various nucleotides. This interaction not only activates the F signals but also produces distinct signatures for each specific component, thereby enabling precise identification and quantification of molecules in evolving samples. We demonstrate the capability of this probe for real-time monitoring of adenosine triphosphate (ATP) hydrolysis and screening potential enzyme inhibitors. These applications highlight the probe's significant potential in enzyme analysis, drug development, and disease diagnostics.
分子转化的实时监测对于生物技术的进步至关重要。在本研究中，我们引入了一种新型的自组装F标记核磁共振（NMR）探针，该探针在与各种核苷酸相互作用时会分解。这种相互作用不仅会激活F信号，还会为每个特定成分产生独特的信号特征，从而能够对不断变化的样品中的分子进行精确鉴定和定量。我们展示了该探针实时监测三磷酸腺苷（ATP）水解和筛选潜在酶抑制剂的能力。这些应用突出了该探针在酶分析、药物开发和疾病诊断方面的巨大潜力。
