Geissinger H D, Yamashiro S, Ackerley C A, Rhodes P A
Scan Electron Microsc. 1985(Pt 4):1573-81.
Mature rabbits were injected intra-muscularly into the hamstring (HS) muscles with doses of 1.66 mg Cu/kg/day, as cupric acetate, in aqueous solution. Similar doses of Na acetate were injected into control rabbits. Skeletal (HS) and cardiac (MYO) muscles were examined by scanning electron microscopy, transmission electron microscopy and a microanalyzer and energy dispersive X-ray (MEDX). Na-injected rabbits showed slight myofibrillar lesions in HS after four injections, and mitochondrial inclusions in MYO were noted after two or seven injections. No Cu was found with MEDX. Cu-injected rabbits showed that the severity of lesions of myofibrillar degeneration and mitochondrial inclusions in HS increased with the number of injections and days the rabbits had been on experiment. After seven days on experiment signs of muscle regeneration were seen. Cu was found first to localize in the nucleus of HS after one day, thereafter it localized in the mitochondria after three and seven days. MYO lesions were found in the mitochondria after six or seven days after Cu-injection. With MEDX less Cu was found than had been found in HS. This Cu first localized in the nucleus of MYO after one day and then disappeared from the tissue altogether.
将成年兔子的腿筋（HS）肌肉进行肌肉注射，以醋酸铜水溶液的形式，剂量为1.66毫克铜/千克/天。将相似剂量的醋酸钠注射到对照兔子体内。通过扫描电子显微镜、透射电子显微镜以及微分析仪和能量色散X射线（MEDX）对骨骼肌（HS）和心肌（MYO）进行检查。注射钠的兔子在四次注射后HS出现轻微肌原纤维病变，在两次或七次注射后MYO出现线粒体包涵体。MEDX未检测到铜。注射铜的兔子显示，HS中肌原纤维变性和线粒体包涵体的病变严重程度随着注射次数和兔子实验天数的增加而增加。实验七天后出现肌肉再生迹象。注射铜一天后首先在HS的细胞核中发现铜，三天和七天后定位于线粒体。注射铜六天或七天后在MYO的线粒体中发现病变。通过MEDX在MYO中发现的铜比在HS中发现的少。这种铜在一天后首先定位于MYO的细胞核，然后完全从组织中消失。
