Microalgae Biota Technology Group, Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; Department of Fisheries, Faculty of Agriculture, University of Maiduguri, P.M.B. 1069, Maiduguri, Borno State, Nigeria.
Microalgae Biota Technology Group, Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Environ Pollut. 2024 Dec 15;363(Pt 1):125103. doi: 10.1016/j.envpol.2024.125103. Epub 2024 Oct 12.
Microplastics (MPs) are widely used and disposed of indiscriminately, posing a potential threat to aquatic life. Herein, Asian seabass (Lates calcarifer) fingerlings were exposed to various concentrations (1, 10 and 100 ppt or g/kg) of dietary polyethylene MPs for 16 days. The results indicated a significant increase in mortality among the fish fed with dietary MPs compared to the control. Furthermore, histological analysis of the liver revealed moderate-to-severe morphological alterations, hepatocyte necrosis and vacuolisation as the concentration gradient of MPs increased. The severity of the alterations was highest at a concentration of 100 ppt, indicating a direct correlation between MP and liver damage. In addition, RNA sequencing and Gene Ontology term enrichment analysis revealed that a total of 4137 genes were significantly differentially expressed, with 1958 upregulated and 2179 downregulated genes. The significantly enriched terms included 'oxidoreductase activity', 'endocytosis', 'mitochondrial', 'immune system process' and 'lipid catabolic process'. Moreover, the Kyoto Encyclopaedia of Genes and Genomes enrichment analysis demonstrated that dietary MPs triggered oxidative stress, immune response and adaptive mechanism pathways in fish. Thus, MPs can induce metabolic disorders in L. calcarifer, highlighting their potential threat to aquatic organisms.
微塑料（MPs）广泛使用且随意丢弃，对水生生物构成潜在威胁。本研究以卵形鲳鲹（Lates calcarifer）鱼苗为实验对象，暴露于不同浓度（1、10 和 100 ppt 或 g/kg）的饮食性聚乙烯 MPs 中 16 天。结果表明，与对照组相比，摄食 MPs 的鱼死亡率显著增加。此外，肝脏组织学分析显示，随着 MPs 浓度梯度的增加，出现了中度至重度的形态改变、肝细胞坏死和空泡化。在 100 ppt 浓度下，改变最为严重，表明 MPs 与肝损伤之间存在直接相关性。此外，RNA 测序和基因本体论术语富集分析表明，共有 4137 个基因显著差异表达，其中 1958 个上调，2179 个下调。显著富集的术语包括“氧化还原酶活性”、“内吞作用”、“线粒体”、“免疫系统过程”和“脂质代谢过程”。此外，京都基因与基因组百科全书富集分析表明，饮食性 MPs 引发了鱼类的氧化应激、免疫反应和适应机制途径。因此，MPs 可导致卵形鲳鲹发生代谢紊乱，突显其对水生生物的潜在威胁。
