Wu Tingyu, Ma Yangguang, Yang Yongyong, Zhang Zhihao, Zhou Jie, Ju Cheng, Zuo Xiaoshuang, Wang Xuankang, Hu Xueyu, Wang Zhe
Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
Xi'an Medical University, Xi'an, Shaanxi, China.
Photodiagnosis Photodyn Ther. 2024 Dec;50:104364. doi: 10.1016/j.pdpdt.2024.104364. Epub 2024 Oct 12.
Spinal cord swelling commonly occurs following SCI. Previous studies suggest that PBM may reduce inflammation and scar formation after SCI. However, whether PBM can alleviate post-spinal cord injury edema and its underlying mechanisms have not yet been reported. This study aims to investigate the effects of PBM on spinal cord swelling in rats following SCI and explore the underlying mechanisms.
A rat model of SCI was established, and the rats received continuous PBM therapy for two weeks. Tissue hydration, motor function, AQP4 expression, and pathological changes in the spinal cord were evaluated at different time points. In vitro, astrocytes were subjected to PBM and treated with either cucurbitacin I or TGN020 following OGD.
The results indicate that PBM reduces tissue swelling in rats with SCI, improves motor function recovery, and inhibits the upregulation of AQP4 and GFAP associated with SCI. In vitro, PBM reduces abnormal activation of the JAK2/STAT3 signaling pathway in astrocytes, leading to decreased AQP4 synthesis and astrocyte activation.
These findings suggest that PBM reduces spinal cord swelling in rats after injury. This effect is associated with the inhibition of JAK2/STAT3 signaling pathway activation in astrocytes and the reduction in AQP4 expression.
脊髓损伤（SCI）后常发生脊髓肿胀。先前的研究表明，低强度脉冲超声（PBM）可能会减轻SCI后的炎症和瘢痕形成。然而，PBM是否能减轻脊髓损伤后的水肿及其潜在机制尚未见报道。本研究旨在探讨PBM对SCI大鼠脊髓肿胀的影响，并探索其潜在机制。
建立大鼠SCI模型，大鼠接受连续两周的PBM治疗。在不同时间点评估脊髓的组织含水量、运动功能、水通道蛋白4（AQP4）表达及病理变化。在体外，对星形胶质细胞进行PBM处理，并在氧糖剥夺（OGD）后用葫芦素I或TGN020处理。
结果表明，PBM可减轻SCI大鼠的组织肿胀，改善运动功能恢复，并抑制与SCI相关的AQP4和胶质纤维酸性蛋白（GFAP）的上调。在体外，PBM可降低星形胶质细胞中JAK2/信号转导子和转录激活子3（STAT3）信号通路的异常激活，导致AQP4合成减少和星形胶质细胞活化。
这些发现表明，PBM可减轻大鼠损伤后的脊髓肿胀。这种作用与抑制星形胶质细胞中JAK2/STAT3信号通路的激活及AQP4表达的降低有关。
