[电针对肥胖大鼠炎症反应及肠道菌群的影响]

[Effects of electroacupuncture on the inflammatory response and intestinal flora in obese rats].

作者信息

Tian Hao-Ran, Zhou Yu-Dian, Lu Da-Min, Yang Shu-Rui, Kang Wen-Wu, Tang Qian, Liang Feng-Xia

机构信息

College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430060, China.

Hubei Shizhen Laboratory, Wuhan 430060.

出版信息

Zhen Ci Yan Jiu. 2024;49(9):949-956. doi: 10.13702/j.1000-0607.20230582.

DOI:10.13702/j.1000-0607.20230582

PMID:39401832

Abstract

OBJECTIVES

To observe the effect of electroacupuncture (EA) on the expressions of high mobility group protein 1(HMGB1) and myeloid differentiation factor 88 (MyD88) in the small intestine and intestinal flora of obese rats, so as to explore the potential mechanism of EA to improve obesity in rats.

METHODS

After 1 week of acclimatization, 10 rats were randomly selected from 50 Wistar male rats as the normal group, and the rest rats were fed with high-fat diet for 8 weeks to establish the obese model. The successfully modeling rats were randomly divided into model group, EA group and sham EA group, with 10 rats in each group. Rats in the EA group were given EA (2 Hz, 1 mA) at "Zhongwan"(CV12), "Guanyuan"(CV4), "Zusanli" (ST36)and "Fenglong"(ST40). Rats in the sham EA group were given shallow stabs at acupoints of the EA group about 5 mm outwardly and the electrodes were clamped without being energized. Both groups were intervened for 10 min each time, 3 times (Monday, Wednesday and Friday) a week for 8 weeks. The body weights of the rats were measured before and after 8 weeks of intervention, respectively. The contents of serum lipopolysaccharide (LPS) and tumor necrosis factor (TNF)-α were measured by ELISA, and the protein and mRNA expressions of HMGB1 and MyD88 in the small intestine were detected by Western blot and real-time quantitative PCR, respectively. 16S rRNA sequencing was performed to determine the relative abundance and diversity of the bacterial flora in the fresh feces of rats.

RESULTS

Compared with the normal group, the body weight, serum LPS and TNF-α contents, small intestinal HMGB1 and MyD88 protein and mRNA expression levels of rats in the model group were significantly increased (<0.01), while the relative abundance of , and was decreased (<0.01), , and was increased (<0.01). Compared with model group, the body weight, serum LPS and TNF-α contents, protein and mRNA expression levels of HMGB1 and MyD88 in both EA and sham EA groups were decreased (<0.01, <0.05), while the relative abundance of , and was increased (<0.01) and , and decreased (<0.01). Comparison between EA group and sham EA group showed that, the contents of LPS and TNF-α in serum of rats in sham EA group were increased (<0.01, <0.05), the relative abundance of , and was lower (<0.05, <0.01), and , and was higher (<0.01).

CONCLUSIONS

EA can reduce the body weight of obese rats, which may be related to the regulation of the structure of intestinal flora and the reduction of inflammatory reactions in the small intestine.

摘要

目的

观察电针（EA）对肥胖大鼠小肠中高迁移率族蛋白1（HMGB1）和髓样分化因子88（MyD88）表达及肠道菌群的影响，以探讨EA改善大鼠肥胖的潜在机制。

方法

适应性喂养1周后，从50只雄性Wistar大鼠中随机选取10只作为正常组，其余大鼠给予高脂饮食8周以建立肥胖模型。将造模成功的大鼠随机分为模型组、EA组和假EA组，每组10只。EA组大鼠在“中脘”（CV12）、“关元”（CV4）、“足三里”（ST36）和“丰隆”（ST40）进行电针治疗（2Hz，1mA）。假EA组大鼠在EA组穴位向外浅刺约5mm，夹上电极但不通电。两组每次干预10min，每周3次（周一、周三和周五），共8周。分别在干预8周前后测量大鼠体重。采用ELISA法检测血清脂多糖（LPS）和肿瘤坏死因子（TNF）-α含量，分别用Western blot和实时定量PCR检测小肠中HMGB1和MyD88的蛋白及mRNA表达。进行16S rRNA测序以确定大鼠新鲜粪便中细菌菌群的相对丰度和多样性。

结果

与正常组相比，模型组大鼠体重、血清LPS和TNF-α含量、小肠HMGB1和MyD88蛋白及mRNA表达水平显著升高（<0.01），而、和的相对丰度降低（<0.01），、和升高（<0.01）。与模型组相比，EA组和假EA组大鼠体重、血清LPS和TNF-α含量、HMGB1和MyD88蛋白及mRNA表达水平均降低（<0.01，<0.05），而、和的相对丰度升高（<0.01），、和降低（<0.01）。EA组与假EA组比较，假EA组大鼠血清LPS和TNF-α含量升高（<0.01，<0.05），、和的相对丰度较低（<0.05，<0.01），、和较高（<0.01）。