[不同穴位组电针刺激对肥胖大鼠脂质代谢及肝脏TLR4/NF-κB信号通路的影响]

[Effect of electroacupuncture stimulation of different acupoint groups on lipid metabolism and liver TLR4/NF-κB signaling in obese rats].

作者信息

Yang Shu-Rui, Zhou Yu-Dian, Chen Rui, Lu Wei, Wu Song, Wang Ya-Yuan, Chen Li, Liang Feng-Xia

机构信息

College of Acupuncture-moxibustion and Orthopedics-traumatology, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430061, China.

Department of Integrated Traditional Chinese and Western Medicine, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022.

出版信息

Zhen Ci Yan Jiu. 2021 Oct 25;46(10):845-50. doi: 10.13702/j.1000-0607.20210522.

DOI:10.13702/j.1000-0607.20210522

PMID:34698458

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) of different acupoint combinations on lipid metabolism and liver Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) signaling in obese rats, so as to explore the specificity of regulatory effects of different acupoints for obesity.

METHODS

Male SD rats were randomly divided into normal control, model, hindlimb acupoint EA ［"Fenglong" (ST40)-"Zusanli" (ST36), hindlimb EA］, abdominal acupoint EA ［("Zhongwan" (CV12)-"Tianshu" (ST25), "Guanyuan" (CV4) - the opposite ST25, abdominal EA］, BiaoBen-acupoint EA ［ST40-ST36, CV12 -CV4, ST25, BiaoBen EA］ groups, with 10 rats in each group. The obesity model was induced by feeding the rats with high fat diet. EA (2 Hz, 1 mA) was applied to the acupoint groups mentioned above for 10 min (per acupoint group), 3 times a week for 8 weeks. After the treatment, the body weight and food intake in each group were recorded. The contents of serum total cholesterol (TC), triglyceride (TG), non-esterified fatty acid (NEFA) were detected by using an automatic biochemical analyzer. The expression levels of TLR4 and NF-κB p65 mRNAs and proteins in the liver tissue were detected by quantitative real-time PCR and Western blot, separately.

RESULTS

Compared with the normal group, the body weight and food intake, serum TC, TG and NEFA contents, hepatic TLR4 and NF-κB p65 protein and gene expression were significantly increased in the model group (<0.05, <0.01). After the treatment, compared with the model group, the body weight, food intake, TC, TG, NEFA, TLR4 and NF-κB p65 protein and mRNA expression were significantly decreased in the hindlimb EA, abdominal EA and BiaoBen EA groups (<0.05, <0.01). Comparison among the 3 treatment groups showed that the serum TC, TG and NEFA contents were significantly lower in the hindlimb EA and BiaoBen EA groups than in the abdominal EA group (<0.01，<0.05). The expression levels of TLR4 and NF-κB p65 mRNAs and proteins were significantly lower in the BiaoBen EA group than in the hindlimb EA and abdominal EA groups (<0.01, <0.05).

CONCLUSION

BiaoBen EA is superior to abdominal EA in improving lipid metabolism in obesity rats, which may be related to its stronger effectiveness in down-regulating hepatic TLR4/NF-κB p65 signaling.

摘要

目的

观察不同穴位组合电针（EA）对肥胖大鼠脂质代谢及肝脏Toll样受体4（TLR4）/核因子κB（NF-κB）信号通路的影响，以探讨不同穴位对肥胖调节作用的特异性。

方法

雄性SD大鼠随机分为正常对照组、模型组、后肢穴位EA组［“丰隆”（ST40）-“足三里”（ST36），后肢EA组］、腹部穴位EA组［（“中脘”（CV12）-“天枢”（ST25），“关元”（CV4）-对侧ST25，腹部EA组］、标本配穴EA组［ST40-ST36，CV12-CV4，ST25，标本EA组］，每组10只。采用高脂饮食诱导大鼠建立肥胖模型。对上述穴位组施加EA（2 Hz，1 mA）10分钟（每个穴位组），每周3次，共8周。治疗后，记录每组大鼠的体重和食量。使用自动生化分析仪检测血清总胆固醇（TC）、甘油三酯（TG）、非酯化脂肪酸（NEFA）含量。分别采用定量实时PCR和Western blot检测肝组织中TLR4和NF-κB p65 mRNA及蛋白表达水平。

结果

与正常组相比，模型组大鼠体重、食量、血清TC、TG和NEFA含量、肝脏TLR4和NF-κB p65蛋白及基因表达均显著升高（<0.05，<0.01）。治疗后，与模型组相比，后肢EA组、腹部EA组和标本配穴EA组大鼠体重、食量、TC、TG、NEFA、TLR4和NF-κB p65蛋白及mRNA表达均显著降低（<0.05，<0.01）。3个治疗组比较，后肢EA组和标本配穴EA组血清TC、TG和NEFA含量显著低于腹部EA组（<0.01，<0.05）。标本配穴EA组TLR4和NF-κB p65 mRNA及蛋白表达水平显著低于后肢EA组和腹部EA组（<0.01，<0.05）。