Raaphorst G P, Azzam E I, Sargent M D, Borsa J
Cancer Res. 1986 Jan;46(1):14-9.
The C3H10T1/2 cell system was used to measure oncogenic transformation after heating and after combined treatments of heat and X-rays. Heat alone at 41.0, 42.0, and 45.0 degrees C caused no transformation. Prolonged heating at 42.0 degrees C, or incubation at 37 degrees C after heating at 45.0 degrees C, resulted in thermotolerance in terms of cell killing, but did not cause transformation. Combined treatments of heat and radiation resulted in small increases in the transformation frequency per viable cell when the two treatments were separated by incubation at 37 degrees C. For simultaneous treatments of heat and X-rays, or treatments immediately following each other, the transformation frequency was about the same as, or lower than, with X-rays alone. When transformation was calculated based on the cells at risk, hyperthermia treatment reduced the X-ray transformation frequency in all cases. Thus hyperthermia may not pose a carcinogenic risk in cancer therapy and could decrease the oncogenic potential of X-rays, depending on the treatment sequence.
使用C3H10T1/2细胞系统来测量加热后以及热与X射线联合处理后的致癌转化情况。单独在41.0、42.0和45.0摄氏度加热不会导致转化。在42.0摄氏度长时间加热，或在45.0摄氏度加热后于37摄氏度孵育，就细胞杀伤而言会产生热耐受性，但不会导致转化。当热与辐射联合处理被37摄氏度孵育隔开时，每个活细胞的转化频率会有小幅增加。对于热与X射线同时处理或紧接着彼此的处理，转化频率与单独使用X射线时大致相同或更低。当基于有风险的细胞计算转化时，在所有情况下热疗都会降低X射线的转化频率。因此，热疗在癌症治疗中可能不会构成致癌风险，并且根据治疗顺序，热疗可能会降低X射线的致癌潜力。
