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基于 VIGS 功能分析在黄瓜中对南方根结线虫抗性基因的遗传剖析。

Genetic dissection of Meloidogyne incognita resistance genes based on VIGS functional analysis in Cucumis metuliferus.

机构信息

State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, 6708 PB, The Netherlands.

出版信息

BMC Plant Biol. 2024 Oct 15;24(1):964. doi: 10.1186/s12870-024-05681-6.

DOI:10.1186/s12870-024-05681-6
PMID:39402446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11476473/
Abstract

The southern root-knot nematode, Meloidogyne incognita, is a highly serious plant parasitic nematode species that causes significant economic losses in various crops, including cucumber (Cucumis sativus L.). Currently, there are no commercial cultivars available with resistance to M. incognita in cucumber. However, the African horned melon (Cucumis metuliferus Naud.), a semi-wild relative of cucumber, has shown high resistance to M. incognita. In this study, we constructed an ultrahigh-density genetic linkage bin-map using low-coverage sequences from an F population generated through the cross between C. metuliferus inbred lines CM3 and CM27. Finally, we identified a QTL (quantitative trait locus, QTL3.1) with a LOD (logarithm of the odds) score of 3.84, explaining 8.4% of the resistance variation. Subsequently, by combining the results of qPCR (quantitative PCR) and VIGS (virus-induced gene silencing), we identified two genes, EVM0025394 and EVM0006042, that are potentially involved in the resistance to M. incognita in CM3. The identification of QTLs and candidate genes in this study serve as a basis for further functional analysis and lay the groundwork for harnessing this resistance trait.

摘要

南方根结线虫（Meloidogyne incognita）是一种高度严重的植物寄生线虫，会给包括黄瓜（Cucumis sativus L.）在内的各种作物造成重大经济损失。目前，黄瓜中还没有具有抗南方根结线虫特性的商业品种。然而，非洲黄瓜（Cucumis metuliferus Naud.）是黄瓜的半野生近缘种，对南方根结线虫表现出高度抗性。在本研究中，我们使用来自非洲黄瓜自交系 CM3 和 CM27 杂交产生的 F1 群体的低覆盖率序列构建了一个超高密度遗传连锁 bin 图谱。最后，我们鉴定出一个 QTL（数量性状位点，QTL3.1），其 LOD（对数优势）得分为 3.84，解释了 8.4%的抗性变异。随后，通过 qPCR（定量 PCR）和 VIGS（病毒诱导基因沉默）的结果结合，我们鉴定出两个可能参与 CM3 抗南方根结线虫的基因 EVM0025394 和 EVM0006042。本研究中 QTL 和候选基因的鉴定为进一步的功能分析奠定了基础，并为利用这种抗性特性奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/d2da3f37658b/12870_2024_5681_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/058302ec57e8/12870_2024_5681_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/a17efcdd8ea6/12870_2024_5681_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/5d23305f8956/12870_2024_5681_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/7104a5d34f29/12870_2024_5681_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/d2da3f37658b/12870_2024_5681_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/058302ec57e8/12870_2024_5681_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/a17efcdd8ea6/12870_2024_5681_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/5d23305f8956/12870_2024_5681_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/7104a5d34f29/12870_2024_5681_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5fc0/11476473/d2da3f37658b/12870_2024_5681_Fig5_HTML.jpg

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