Lew A M, Maloy W L, Coligan J E
J Immunol. 1986 Jan;136(1):254-8.
Q10 is a class I molecule previously proven to be secreted rather than membrane bound. To measure the amount of Q10 in various mouse sera, a quantitative Western blot assay was developed. Q10 was the only class I molecule detectable in mouse sera. It occurs as a high m.w. complex of 200,000 to 300,000. The amount of Q10 in serum varies among different mouse strains and is controlled by a region telomeric to H-2S. Mice of the f haplotype do not express Q10, but all other mice examined (20 strains) with inbred or wild-derived H-2 haplotypes do. The H-2 haplotypes rank according to their levels of Q10 as follows: z, s greater than k, b greater than d, q greater than f; and the actual values range from to 60 micrograms/ml to undetectable levels in serum. In some strains the levels are higher in males than in females. The levels increase with age and decrease during pregnancy but not during lactation. There is a dramatic decrease after the injection of irritants or syngeneic tumor transplantation, but allostimulation has no effect on Q10 levels. The possible significance of this soluble class I molecule is discussed in the light of our findings.
Q10是一种I类分子，先前已证明它是分泌型而非膜结合型。为了测量各种小鼠血清中Q10的含量，开发了一种定量蛋白质印迹分析方法。Q10是小鼠血清中唯一可检测到的I类分子。它以200,000至300,000的高分子量复合物形式存在。血清中Q10的含量在不同小鼠品系中有所不同，并受H - 2S端粒区域的控制。f单倍型的小鼠不表达Q10，但所有其他检测的具有近交或野生来源H - 2单倍型的小鼠（20个品系）都表达。H - 2单倍型根据其Q10水平排序如下：z、s大于k、b大于d、q大于f；血清中的实际值范围从60微克/毫升到无法检测到的水平。在某些品系中，雄性的水平高于雌性。水平随年龄增加而升高，在怀孕期间降低，但在哺乳期不降低。注射刺激物或同基因肿瘤移植后会显著降低，但异体刺激对Q10水平没有影响。根据我们的发现讨论了这种可溶性I类分子的可能意义。
