• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于高通量电荷变体分析的超音速IEF方法的开发。

Development of the SupersonicIEF Method for High-Throughput Charge Variant Analysis.

作者信息

McElroy Will, Heger Christopher D

机构信息

Applications Science, ProteinSimple, a Bio-Techne Brand, San Jose, California, USA.

出版信息

Electrophoresis. 2024 Nov;45(21-22):1968-1975. doi: 10.1002/elps.202400117. Epub 2024 Oct 14.

DOI:10.1002/elps.202400117
PMID:39402844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11662200/
Abstract

The analysis of biopharmaceuticals for charge variants occurs from early-stage samples through formulation and process-development optimization. Higher throughput methods allow increased analysis of these samples to facilitate greater understanding of the samples and to better optimize their production and formulation. To enable higher throughput charge variant analysis, a new, rapid platform imaged capillary isoelectric focusing (icIEF) method was optimized to be two to three times faster than standard methods.

摘要

对生物制药电荷变体的分析贯穿于从早期样品到制剂及工艺开发优化的整个过程。更高通量的方法能够增加对这些样品的分析，从而有助于更深入地了解样品，并更好地优化其生产和制剂。为实现更高通量的电荷变体分析，一种新型快速平台成像毛细管等电聚焦（icIEF）方法得到了优化，其速度比标准方法快两到三倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/577ef1d94e9f/ELPS-45--g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/6af633e0ffaa/ELPS-45--g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/64792febaeeb/ELPS-45--g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/a86ac78e655c/ELPS-45--g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/1ac9ff3b9218/ELPS-45--g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/aa37a9f813fe/ELPS-45--g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/ce81f90cffb9/ELPS-45--g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/577ef1d94e9f/ELPS-45--g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/6af633e0ffaa/ELPS-45--g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/64792febaeeb/ELPS-45--g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/a86ac78e655c/ELPS-45--g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/1ac9ff3b9218/ELPS-45--g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/aa37a9f813fe/ELPS-45--g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/ce81f90cffb9/ELPS-45--g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df79/11662200/577ef1d94e9f/ELPS-45--g003.jpg

相似文献

1
Development of the SupersonicIEF Method for High-Throughput Charge Variant Analysis.用于高通量电荷变体分析的超音速IEF方法的开发。
Electrophoresis. 2024 Nov;45(21-22):1968-1975. doi: 10.1002/elps.202400117. Epub 2024 Oct 14.
2
Development of a novel, high-throughput imaged capillary isoelectric focusing-Western method to characterize charge heterogeneity of monoclonal antibody heavy and light chains.开发一种新型高通量成像毛细管等电聚焦-免疫印迹方法，用于表征单克隆抗体重链和轻链的电荷异质性。
MAbs. 2024 Jan-Dec;16(1):2429414. doi: 10.1080/19420862.2024.2429414. Epub 2024 Nov 15.
3
Imaged capillary isoelectric focusing (icIEF) tandem high resolution mass spectrometry for charged heterogeneity of protein drugs in biopharmaceutical discovery.成像毛细管等电聚焦（icIEF）串联高分辨率质谱法用于生物制药研发中蛋白质药物的电荷异质性分析
J Pharm Biomed Anal. 2023 Feb 5;224:115178. doi: 10.1016/j.jpba.2022.115178. Epub 2022 Nov 21.
4
Imaged capillary isoelectric focusing method development for charge variants of high DAR ADCs.用于高载药比 ADC 电荷变异体的成像毛细管等电聚焦方法的开发。
Anal Chim Acta. 2024 Nov 1;1328:343176. doi: 10.1016/j.aca.2024.343176. Epub 2024 Aug 28.
5
Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing.基于成像毛细管等电聚焦技术的三种常见蛋白治疗药物的荷质异质性分析平台方法。
Methods Mol Biol. 2021;2261:93-103. doi: 10.1007/978-1-0716-1186-9_8.
6
Fractionation and online mass spectrometry based on imaged capillary isoelectric focusing (icIEF) for characterizing charge heterogeneity of therapeutic antibody.基于成像毛细管等电聚焦（icIEF）的分级分离和在线质谱分析用于表征治疗性抗体的电荷异质性
Anal Methods. 2023 Jan 26;15(4):411-418. doi: 10.1039/d2ay01670b.
7
A platform method for charge heterogeneity characterization of fusion proteins by icIEF.利用等电聚焦-离子淌度分离技术的平台方法对融合蛋白的荷质比异质性进行表征。
Anal Biochem. 2022 Feb 1;638:114505. doi: 10.1016/j.ab.2021.114505. Epub 2021 Nov 29.
8
Determination of protein charge variants with (imaged) capillary isoelectric focusing and capillary zone electrophoresis.采用（成像）毛细管等电聚焦和毛细管区带电泳法测定蛋白质电荷变体。
Electrophoresis. 2018 Oct;39(20):2492-2511. doi: 10.1002/elps.201800079. Epub 2018 Jun 19.
9
Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Levels.毛细管等电聚焦：一种用于分离和在线表征完整和亚基水平单克隆抗体电荷变异体的质谱方法。
Methods Mol Biol. 2022;2500:55-65. doi: 10.1007/978-1-0716-2325-1_6.
10
CE methods for charge variant analysis of mAbs and complex format biotherapeutics.CE 方法用于单抗和复杂结构生物治疗药物的电荷变异分析。
Electrophoresis. 2024 Aug;45(15-16):1295-1306. doi: 10.1002/elps.202300170. Epub 2024 Jan 17.

引用本文的文献

1
Evaluation of a cIEF Fractionation Workflow for Offline MS Analysis of Charge Variants of the Monoclonal Antibody Matuzumab.用于单克隆抗体美妥珠单抗电荷变体离线质谱分析的毛细管等速电泳分离工作流程评估
Electrophoresis. 2025 Feb;46(3-4):240-249. doi: 10.1002/elps.8108. Epub 2025 Feb 18.

本文引用的文献

1
Development and Qualification of Analytical Methods to Support Low Concentration Drug Product in-use Studies.支持低浓度药物产品在使用研究的分析方法的开发和验证。
J Pharm Sci. 2024 Mar;113(3):604-615. doi: 10.1016/j.xphs.2023.09.011. Epub 2023 Sep 25.
2
Biopharmaceutical Manufacturing: Historical Perspectives and Future Directions.生物制药制造：历史视角与未来方向
Annu Rev Chem Biomol Eng. 2022 Jun 10;13:141-165. doi: 10.1146/annurev-chembioeng-092220-125832. Epub 2022 Mar 17.
3
A platform method for charge heterogeneity characterization of fusion proteins by icIEF.
利用等电聚焦-离子淌度分离技术的平台方法对融合蛋白的荷质比异质性进行表征。
Anal Biochem. 2022 Feb 1;638:114505. doi: 10.1016/j.ab.2021.114505. Epub 2021 Nov 29.
4
Capillary isoelectric focusing with UV fluorescence imaging detection enables direct charge heterogeneity characterization of erythropoietin drug products.毛细管等电聚焦与紫外荧光成像检测相结合，可直接对红细胞生成素药物产品进行电荷异质性表征。
J Chromatogr A. 2021 Apr 26;1643:462043. doi: 10.1016/j.chroma.2021.462043. Epub 2021 Mar 15.
5
Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing.基于成像毛细管等电聚焦技术的三种常见蛋白治疗药物的荷质异质性分析平台方法。
Methods Mol Biol. 2021;2261:93-103. doi: 10.1007/978-1-0716-1186-9_8.
6
Structure, heterogeneity and developability assessment of therapeutic antibodies.治疗性抗体的结构、异质性和可开发性评估。
MAbs. 2019 Feb/Mar;11(2):239-264. doi: 10.1080/19420862.2018.1553476. Epub 2018 Dec 17.
7
Quantitation of CRM197 using imaged capillary isoelectric focusing with fluorescence detection and capillary Western.使用带荧光检测的成像毛细管等电聚焦和毛细管免疫印迹法对CRM197进行定量分析。
Anal Biochem. 2017 Oct 1;534:19-23. doi: 10.1016/j.ab.2017.06.013. Epub 2017 Jun 27.
8
Robustness of iCIEF methodology for the analysis of monoclonal antibodies: an interlaboratory study.iCIEF 方法分析单克隆抗体的稳健性：一项实验室间研究。
J Sep Sci. 2012 Nov;35(22):3124-9. doi: 10.1002/jssc.201200633. Epub 2012 Oct 15.
9
Analysis of charge heterogeneities in mAbs using imaged CE.使用成像毛细管电泳分析单克隆抗体中的电荷异质性。
Electrophoresis. 2009 Mar;30(5):714-22. doi: 10.1002/elps.200800636.
10
Determination of the operational pH value of a buffering membrane by an isoelectric trapping separation of a carrier ampholyte mixture.通过载体两性电解质混合物的等电聚焦分离测定缓冲膜的操作pH值。
Electrophoresis. 2008 Mar;29(5):1077-81. doi: 10.1002/elps.200700698.