Chatterjee Gaurav, He Rong, Patkar Nikhil, Viswanatha David, Langerak Anton W
Hematopathology Department, ACTREC, Tata Memorial Centre, Mumbai, India.
Division of Hematopathology, Mayo Clinic, Rochester, MN, USA.
Histopathology. 2025 Jan;86(1):38-57. doi: 10.1111/his.15332. Epub 2024 Oct 15.
Here we review the 'what and how' of molecular techniques used in the context of haematopathological diagnostics of both lymphoid and myeloid neoplasms. Keeping in mind that the required resources for molecular testing are not universally available, we will not only discuss novel and emerging techniques that allow more high-throughput and sophisticated analyses of lymphoid and myeloid neoplasms, but also the more classical, low-cost alternatives and even some workarounds for molecular testing approaches. In this review we also address other key aspects around molecular techniques for haematopatholgy diagnostics, including preanalytics, data interpretation, and data management, bioinformatics, and interlaboratory precision and performance evaluation.
在此，我们回顾了用于淋巴样和髓样肿瘤血液病理学诊断的分子技术的“内容与方法”。鉴于并非普遍具备分子检测所需的资源，我们不仅会讨论能够对淋巴样和髓样肿瘤进行更高通量、更精细分析的新型和新兴技术，还会讨论更经典、低成本的替代方法，甚至一些分子检测方法的变通方案。在本综述中，我们还将探讨血液病理学诊断分子技术的其他关键方面，包括分析前、数据解读、数据管理、生物信息学以及实验室间的精密度和性能评估。
