Johnson R P, Sar M, Stumpf W E
Neurosci Lett. 1979 Oct;14(2-3):321-6. doi: 10.1016/0304-3940(79)96168-8.
A technique for the localization of catecholamines and enkephalin in the same tissue section is described. Sections from rat brains prepared according to the Falck-Hillarp technique were examined by dark field fluorescence microscopy and then processed for immunocytochemistry for leucine-enkephalin (leu-enk) using the unlabeled antibody technique. In the medial hypothalamus the distribution of the catecholamines and enkephalin is complementary, while in the lateral hypothalamus the patterns are similar but not identical. Enkephalin terminals are also observed in and around the locus coeruleus, while enkephalin cell bodies are adjacent to the nucleus.
本文描述了一种在同一组织切片中定位儿茶酚胺和脑啡肽的技术。按照Falck-Hillarp技术制备的大鼠脑切片，先用暗视野荧光显微镜检查，然后采用未标记抗体技术进行亮氨酸脑啡肽（亮脑啡肽）的免疫细胞化学处理。在内侧下丘脑，儿茶酚胺和脑啡肽的分布是互补的，而在外侧下丘脑，其模式相似但不完全相同。在蓝斑及其周围也观察到脑啡肽终末，而脑啡肽细胞体则靠近细胞核。
