He Ming, Long Gui-Jun, Feng Hong-Yan, Zhao Ya-Qin, Zhou Yang-Yuntao, Zhang Meng-Qi, Ma Yun-Feng, Gong Lang-Lang, Hull J Joe, Zotti Moises J, Dewer Youssef, He Peng, Smagghe Guy
State Key Laboratory of Green Pesticide; Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang, China.
Pest Management and Biocontrol Research Unit, US Arid Land Agricultural Research Center, USDA-Agricultural Research Services, Maricopa, Arizona, USA.
Pest Manag Sci. 2024 Oct 15. doi: 10.1002/ps.8474.
The takeout (TO) gene family impacts diverse physiological and behavioral functions in insects, yet specific olfactory-associated roles for the family have yet to be fully elucidated. To provide insights into TO function in rice planthoppers, the genomes of three rice planthoppers (white-backed planthopper, brown planthopper and small brown planthopper) were searched for TO homologs and their degree of conservation assessed via chromosomal localization, exon-intron boundaries, phylogenetic relationships and protein domains/motifs. We performed a tissue-specific expression analysis of the 20 TO genes in the white-backed planthopper (WBPH, Sogatella furcifera) and found that SfTO17 is enriched in adult antennae. RNAi-mediated knockdown of SfTO17 impaired WBPH olfaction and reduced host-seeking responses following exposure to rice plants. The binding profile of β-ionone, hexyl benzoate and benzyl benzoate with recombinant SfTO17 was evaluated via competitive fluorescence binding assays. Conformational prediction of SfTO17 coupled with molecular docking analyses revealed several amino acid residues potentially critical for odorant binding. This study demonstrates the olfactory function of SfTO17 in WBPH and highlights its potential as a target for controlling this rice pest. © 2024 Society of Chemical Industry.
外卖（TO）基因家族影响昆虫的多种生理和行为功能，但该家族在嗅觉方面的具体作用尚未完全阐明。为深入了解TO基因在稻飞虱中的功能，我们在三种稻飞虱（白背飞虱、褐飞虱和灰飞虱）的基因组中搜索TO同源物，并通过染色体定位、外显子 - 内含子边界、系统发育关系以及蛋白质结构域/基序评估它们的保守程度。我们对白背飞虱（Sogatella furcifera）中的20个TO基因进行了组织特异性表达分析，发现SfTO17在成虫触角中富集。RNA干扰介导的SfTO17基因敲低损害了白背飞虱的嗅觉，并降低了其在接触水稻植株后的寄主寻找反应。通过竞争性荧光结合试验评估了β - 紫罗兰酮、苯甲酸己酯和苯甲酸苄酯与重组SfTO17的结合情况。SfTO17的构象预测与分子对接分析表明，有几个氨基酸残基可能对气味结合至关重要。本研究证明了SfTO17在白背飞虱中的嗅觉功能，并突出了其作为控制这种水稻害虫靶点的潜力。© 2024化学工业协会。
