Yang Di, Wang Weiliang, Fang Zhengfu, Wu Simin, Chen Lili, Chen Jie, Zhang Wensong, Wang Feilong, Sun Tianxiao, Xiang Lin, Wang Yanping, Luo Hong, Chan Zhulong
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.
Hubei Hongshan Laboratory, Wuhan, China.
Plant Cell Environ. 2025 Feb;48(2):1115-1129. doi: 10.1111/pce.15211. Epub 2024 Oct 15.
The phospholipase Ds (PLDs) are crucial for cellular signalling and play roles in plant abiotic stress response. In this study, we identified 12 PLD genes from the genome data of perennial ryegrass (Lolium perenne), which is widely used as forage and turfgrass. Among them, LpPLDδ3 was significantly repressed by ABA treatment, and induced by drought stress and heat stress treatments. The ectopic overexpression (OE) of LpPLDδ3 in Arabidopsis enhanced plant tolerance to osmotic and heat stress as demonstrated by an increased survival rate and reduced malondialdehyde (MDA) accumulation and electrolyte leakage (EL). Arabidopsis endogenous ABA RESPONSIVE ELEMENT BINDING FACTORs (ABFs) and heat stress responsive genes were elevated in LpPLDδ3 OE lines under osmotic and heat stress treatments. Additionally, overexpression of LpPLDδ3 in perennial ryegrass protoplasts could increase heat stress tolerance and elevate expression level of heat stress responsive genes. Moreover, LpABF2 and LpABF4 depressed the LpPLDδ3 expression by directly binding to its ABRE core-binding motif of promoter region. In summary, LpPLDδ3 was repressed by LpABF2 and LpABF4 and positively involved in perennial ryegrass osmotic and heat stress responses.
磷脂酶D（PLD）对细胞信号传导至关重要，并在植物非生物胁迫响应中发挥作用。在本研究中，我们从多年生黑麦草（Lolium perenne）的基因组数据中鉴定出12个PLD基因，多年生黑麦草被广泛用作饲料和草坪草。其中，LpPLDδ3在脱落酸（ABA）处理下显著受抑制，而在干旱胁迫和热胁迫处理下被诱导。LpPLDδ3在拟南芥中的异位过表达（OE）增强了植物对渗透胁迫和热胁迫的耐受性，表现为存活率提高、丙二醛（MDA）积累减少和电解质渗漏（EL）降低。在渗透胁迫和热胁迫处理下，拟南芥内源性ABA响应元件结合因子（ABF）和热胁迫响应基因在LpPLDδ3过表达株系中上调。此外，LpPLDδ3在多年生黑麦草原生质体中的过表达可提高热胁迫耐受性并提高热胁迫响应基因的表达水平。此外，LpABF2和LpABF4通过直接结合其启动子区域的ABRE核心结合基序抑制LpPLDδ3的表达。总之，LpPLDδ3受LpABF2和LpABF4抑制，并正向参与多年生黑麦草的渗透胁迫和热胁迫响应。
