Moscow Center for Advanced Studies, Kulakova Str. 20, Moscow 123592, Russia.
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, Moscow 125315, Russia.
Cells. 2024 Sep 26;13(19):1617. doi: 10.3390/cells13191617.
Differentiation approaches to obtain mesenchymal stem cells (MSCs) have gradually developed over the last few decades. The problem is that different protocols give different MSC types, making further research difficult. Here, we tried three different approaches to differentiate embryonic stem cells (ESCs) from early mesoderm to MSCs using serum-containing or xeno-free differentiation medium and observed differences in the cells' morphology, doubling rate, ability to form colonies, surface marker analysis, and multilineage differentiation potential of the obtained cell lines. We concluded that the xeno-free medium best fits the criteria of MSCs' morphology, growth kinetics, and surface marker characterization. In contrast, the serum-containing medium gives better potential for further MSC differentiation into osteogenic, chondrogenic, and adipogenic lineages.
过去几十年来，获得间充质干细胞（MSCs）的分化方法逐渐发展起来。问题是，不同的方案会产生不同类型的 MSC，这使得进一步的研究变得困难。在这里，我们尝试了三种不同的方法，使用含血清或无动物血清的分化培养基，从早期中胚层分化胚胎干细胞（ESCs）为 MSCs，并观察细胞形态、倍增率、集落形成能力、表面标记分析以及获得的细胞系的多能分化潜能的差异。我们得出结论，无动物血清培养基最符合 MSC 形态、生长动力学和表面标记特征的标准。相比之下，含血清培养基为 MSC 进一步分化为成骨细胞、软骨细胞和成脂细胞谱系提供了更好的潜力。
