Department of Hydrobiology and Protection of Ecosystems, University of Life Sciences in Lublin, Dobrzańskiego 37, 20-262 Lublin, Poland.
The Neurotox Lab, School of Science, RMIT University, Plenty Road, P.O. Box 71, Bundoora, VIC 3083, Australia.
Environ Sci Technol. 2024 Oct 29;58(43):19199-19210. doi: 10.1021/acs.est.4c07823. Epub 2024 Oct 15.
This study aimed to assess the behavioral responses (immobilization, horizontal and vertical motility, and response to light) of larvae exposed to individual cyanobacterial metabolites aeruginosin 98B (AER-B), anabaenopeptin-B (ANA-B), and cylindrospermopsin (CYL), and their binary and ternary mixtures. The investigation revealed that single metabolites ANA-B and CYL exhibited the highest potency in immobilizing the larvae. Notably, the binary mixture AER-B+CYL induced a remarkably strong synergistic interaction, while other tested binary and ternary mixtures demonstrated antagonistic effects. Both individual metabolites and their mixtures led to a decrease in larval movement speed, with the AER-B+CYL combination showing a very synergistic effect, and strong antagonistic interactions between the oligopeptides in the ternary mixture. Conversely, while AER-B and the binary mixture ANA-B+CYL stimulated vertical movement, other single metabolites and binary and ternary mixtures decreased this parameter. Antagonistic interactions were observed in all mixtures. ANA-B emerged as the most potent inhibitor, yet all tested metabolites and their mixtures decreased larval response to light, displaying synergistic interactions, except for the AER-B+ANA-B mixture at 250 μg L + 250 μg L. These findings underscore the sensitivity of larvae behavioral parameters as indicators of environmental stressors and mixtures. Consequently, they are recommended for assessing toxic effects induced by cyanobacterial products and other bioactive chemicals.
本研究旨在评估暴露于单个蓝藻代谢物鱼腥藻肽-B（AER-B）、阿那巴肽-B（ANA-B）和节旋藻毒素（CYL）及其二元和三元混合物的幼虫的行为反应（固定、水平和垂直运动以及对光的反应）。研究表明，单一代谢物 ANA-B 和 CYL 对幼虫的固定能力最强。值得注意的是，二元混合物 AER-B+CYL 表现出极强的协同相互作用，而其他测试的二元和三元混合物则表现出拮抗作用。单一代谢物及其混合物均导致幼虫运动速度下降，AER-B+CYL 组合表现出极强的协同作用，而三元混合物中的寡肽之间存在强烈的拮抗相互作用。相反，虽然 AER-B 和二元混合物 ANA-B+CYL 刺激垂直运动，但其他单一代谢物和二元及三元混合物则降低了这一参数。所有混合物均表现出拮抗相互作用。ANA-B 是最有效的抑制剂，但所有测试的代谢物及其混合物均降低了幼虫对光的反应，表现出协同相互作用，除了 AER-B+ANA-B 混合物在 250 μg L + 250 μg L 时。这些发现强调了幼虫行为参数作为环境应激源和混合物的敏感指标的重要性。因此，它们被推荐用于评估蓝藻产物和其他生物活性化学物质引起的毒性效应。
