Department of Anatomy, Yonsei University College of Medicine, Seoul, Republic of Korea; Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea.
Department of Anatomy, Yonsei University College of Medicine, Seoul, Republic of Korea.
J Hazard Mater. 2024 Dec 5;480:136100. doi: 10.1016/j.jhazmat.2024.136100. Epub 2024 Oct 10.
Ingested microplastics (MPs) can accumulate throughout whole body, which may induce the dysfunction of immune system. However, it remains unclear how MP exposure affects innate immune responses at the cellular level. We found that mouse neutrophils strongly bind and then engulf polystyrene MPs. This interaction leads to proinflammatory state of neutrophils and eventually results in apoptotic cell death through toll-like receptor signaling pathway in a bacteria-recognition mimetic manner. Moreover, our data verified that orally administered polystyrene MPs reach various organs in mice, where they are interacted with and endocytosed by neutrophils. We confirmed that human neutrophils also strongly bind and internalize polystyrene MPs. Additionally, RNA sequencing analysis of polystyrene MPs-exposed human neutrophils showed the upregulation of cell death-related function. Therefore, the accumulated MPs may exacerbate inflammatory immune response by disrupting neutrophil function. These results provide novel insight into the adverse responses of neutrophils induced by MP exposure.
摄入的微塑料（MPs）可以在全身积累，这可能导致免疫系统功能障碍。然而，目前尚不清楚 MPs 暴露如何在细胞水平上影响先天免疫反应。我们发现，小鼠中性粒细胞强烈结合并吞噬聚苯乙烯 MPs。这种相互作用导致中性粒细胞的促炎状态，并最终通过 Toll 样受体信号通路以细菌识别模拟的方式导致细胞凋亡死亡。此外，我们的数据证实，口服给予的聚苯乙烯 MPs 到达小鼠的各种器官，在那里它们与中性粒细胞相互作用并被内吞。我们证实人类中性粒细胞也强烈结合并内化聚苯乙烯 MPs。此外，暴露于聚苯乙烯 MPs 的人中性粒细胞的 RNA 测序分析显示细胞死亡相关功能上调。因此，积累的 MPs 通过破坏中性粒细胞功能可能加剧炎症免疫反应。这些结果为 MPs 暴露引起的中性粒细胞的不良反应提供了新的见解。
