Instituto de Física Aplicada, CCT San Luis, CONICET, Argentina; Área de Tecnología Química y Biotecnología, Departamento de Química, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Ejército de los Andes 950, San Luis CP 5700, Argentina.
Instituto Nacional de Tecnología Industrial, INTI San Luis, INTI, Argentina; Área de Básicas Agronómicas, Departamento de Ciencias Agropecuarias, Facultad de Ingeniería y Ciencias Agropecuarias, Universidad Nacional de San Luis, Ruta Prov. N° 55 (Ex. 148) Extremo Norte, Villa Mercedes CP 5730, Argentina.
Int J Food Microbiol. 2025 Jan 2;426:110934. doi: 10.1016/j.ijfoodmicro.2024.110934. Epub 2024 Oct 9.
The aim of this work is the application of pectin coatings containing Cryptococcus laurentii as a method of biocontrol of Penicillium expansum for postharvest protection of apples. For this purpose, the yeast was incorporated into a pectin matrix, and its viability and biocontrol activity in vitro and in vivo against P. expansum was evaluated over time. In addition, the influence of the sterilization process on coating thickness was studied. Results showed that pectin coating with C. laurentii enhanced mycelial growth inhibition in vitro studies, while no significant differences were observed in disease incidence and severity reduction in vivo studies. The sterilization process reduced the viscosity of the pectin solution, resulting in coating thicknesses ranging from 0.5 to 1 μm. As a general evaluation, in vitro and in vivo, biocontrol assays were useful in demonstrating better postharvest protection of the yeast at 7 °C concerning 25 °C.
本工作的目的是应用含毕赤酵母（Cryptococcus laurentii）的果胶涂层作为采后保护苹果的青霉（Penicillium expansum）生物防治方法。为此，将酵母掺入果胶基质中，并评估其在体外和体内随时间推移对青霉的生存能力和生物防治活性。此外，还研究了灭菌过程对涂层厚度的影响。结果表明，含毕赤酵母的果胶涂层可增强体外研究中的菌丝生长抑制作用，而体内研究中对疾病发生率和严重程度的降低没有观察到显著差异。灭菌过程降低了果胶溶液的粘度，导致涂层厚度在 0.5 到 1 微米之间。总的来说，体外和体内生物防治试验表明，与 25°C 相比，7°C 下的酵母对采后的保护效果更好。
