School of Environmental Engineering, Jiangsu Key Laboratory of Industrial Pollution Control and Resource Reuse, Xuzhou University of Technology, Xuzhou 221018, China; Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
School of Environmental Engineering, Jiangsu Key Laboratory of Industrial Pollution Control and Resource Reuse, Xuzhou University of Technology, Xuzhou 221018, China; School of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China.
Aquat Toxicol. 2024 Nov;276:107118. doi: 10.1016/j.aquatox.2024.107118. Epub 2024 Oct 10.
Tire wear particles (TWP) represent a distinctive form of microplastics (MPs) that are widely distributed in aquatic ecosystems. However, the toxicity of various types of TWP on phytoplankton remain to be further explored. Thus, three different TWPs originating from replaced bicycle, car, and electro-mobile tire (marked as BTWP, CTWP, and ETWP) were selected and their long-term biological influences on Chlorella vulgaris were investigated. Results demonstrated TWPs showed a concentration-dependent growth promotion of Chlorella vulgaris, with a maximum promotion rate reached to 40.51 % (10 mg/L, 10 d), 23.5 % (80 mg/L, 12 d), and 28.7 % (20 mg/L, 12 d) in the presence of BTWP, CTWP and ETWP, respectively. Meanwhile, TWPs could stimulate the secretion of EPS and induce oxidative stress. EPS analysis revealed the increase of polysaccharides could protect the cell from the direct contact with TWP particles. Moreover, the increased concentration of EPS also helps to induce the settlement of TWP and reduce the leachate release. The release of TWP into the environment could act as an accelerator for the growth of Chlorella vulgaris, which might further change the normal physicochemical behaviors of algae colony in aquatic system. Our findings provide new insights into the toxicity mechanism of TWPs on freshwater algae and valuable data on environmental risk assessment of TWPs.
轮胎磨损颗粒（Tire wear particles，TWP）代表一种广泛分布于水生生态系统中的独特微塑料（Microplastics，MPs）形式。然而，各种类型的 TWP 对浮游植物的毒性仍需进一步探索。因此，选择了三种源自更换自行车、汽车和电动自行车轮胎的不同类型的 TWP（分别标记为 BTWP、CTWP 和 ETWP），并研究了它们对普通小球藻（Chlorella vulgaris）的长期生物学影响。结果表明，TWP 对普通小球藻表现出浓度依赖性的生长促进作用，在 BTWP、CTWP 和 ETWP 存在的情况下，最大促进率分别达到 40.51%（10mg/L，10d）、23.5%（80mg/L，12d）和 28.7%（20mg/L，12d）。同时，TWP 可以刺激 EPS 的分泌并诱导氧化应激。EPS 分析表明，多糖的增加可以保护细胞免受 TWP 颗粒的直接接触。此外，EPS 浓度的增加还有助于诱导 TWP 的沉降并减少浸出物的释放。TWP 释放到环境中可能会加速普通小球藻的生长，这可能进一步改变水生系统中藻类群体的正常物理化学行为。我们的研究结果为 TWP 对淡水藻类的毒性机制提供了新的见解，并为 TWP 的环境风险评估提供了有价值的数据。
