School of Health & Social Care, Shanghai Urban Construction Vocational College, Shanghai, 201415, China.
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China.
Talanta. 2025 Jan 1;282:127030. doi: 10.1016/j.talanta.2024.127030. Epub 2024 Oct 10.
Nanoplastics are prevalent in the environment and emerging evidence suggests they can induce organ injury by activating oxidative stress. Given that both nanoplastics and Zn levels are intertwined with oxidative stress, it is crucial to investigate the influence of nanoplastics on the level of labile Zn and get a better understanding of their cytotoxicity mechanisms. At the organelle level, the Golgi apparatus plays an active role in stress responses. In this study, we synthesized Golgi-Zn, the first ratiometric fluorescence nanosensor with Golgi apparatus targeting ability for monitoring of Zn. This nanosensor demonstrated high sensitivity and selectivity as well as robust pH stability for Zn sensing. The ratio of the two fluorescence signals of Golgi-Zn showed a good linearity with Zn concentration in the range of 0.5-10 μM, achieving a limit of detection of ∼72.4 nM. Furthermore, the nanosensor exhibited low cytotoxicity and effectively targeted the Golgi apparatus. Leveraging these fascinating features, we successfully applied Golgi-Zn for visualizing exogenous and endogenous Zn levels in the Golgi apparatus. Moreover, with the help of Golgi-Zn, we found that nanoplastics stimulation could increase the level of Zn in the Golgi apparatus.
纳米塑料在环境中普遍存在，新出现的证据表明，它们可以通过激活氧化应激诱导器官损伤。鉴于纳米塑料和锌水平都与氧化应激交织在一起，因此研究纳米塑料对不稳定锌水平的影响，并更好地了解它们的细胞毒性机制至关重要。在细胞器水平上，高尔基体在应激反应中发挥着积极的作用。在本研究中，我们合成了高尔基体-Zn，这是第一个具有高尔基体靶向能力的比率荧光纳米传感器，用于监测锌。该纳米传感器在锌感测方面表现出高灵敏度和选择性以及稳健的 pH 稳定性。高尔基体-Zn 的两个荧光信号的比值与 0.5-10 μM 范围内的锌浓度呈良好的线性关系，检测限约为 72.4 nM。此外，该纳米传感器表现出低细胞毒性，并能有效地靶向高尔基体。利用这些迷人的特性，我们成功地应用高尔基体-Zn 来可视化高尔基体中外源性和内源性锌水平。此外，借助高尔基体-Zn，我们发现纳米塑料刺激可以增加高尔基体中锌的水平。
