Moscow Institute of Physics and Technology (MIPT), 9 Institutsky lane, Dolgoprudny, 141700, Russian Federation.
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", 5 Prospekt Nauki, Pushchino, 142290, Russian Federation.
Sci Rep. 2024 Oct 15;14(1):24185. doi: 10.1038/s41598-024-75127-4.
The primary role of telomerase is the lengthening of telomeres. Nonetheless, emerging evidence highlights additional functions of telomerase outside of the nucleus. Specifically, its catalytic subunit, TERT (Telomerase Reverse Transcriptase), is detected in the cytosol and mitochondria. Several studies have suggested an elevation in TERT concentration within mitochondria in response to oxidative stress. However, the origin of this mitochondrial TERT, whether transported from the nucleus or synthesized de novo, remains uncertain. In this study, we investigate the redistribution of TERT, labeled with a SNAP-tag, in response to oxidative stress using laser scanning fluorescence microscopy. Our findings reveal that, under our experimental conditions, there is no discernible transport of TERT from the nucleus to the mitochondria due to oxidative stress.
端粒酶的主要作用是延长端粒。然而，新出现的证据强调了端粒酶在核外的其他功能。具体来说，它的催化亚基，TERT（端粒酶逆转录酶），在细胞质和线粒体中被检测到。几项研究表明，在氧化应激的情况下，线粒体中端粒酶的浓度升高。然而，这种线粒体 TERT 的来源，是从核内运输而来还是从头合成，仍不确定。在这项研究中，我们使用激光扫描荧光显微镜研究了在氧化应激下，用 SNAP 标签标记的 TERT 的重新分布。我们的发现表明，在我们的实验条件下，由于氧化应激，端粒酶不会从核内明显转运到线粒体。
