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LncRNA 通过促进 MYH9 的泛素化促进鸡 LMH 细胞中的脂质沉积。

LncRNA Facilitates Lipid Deposition by Promoting the Ubiquitination of MYH9 in Chicken LMH Cells.

机构信息

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.

Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Henan Agricultural University, Zhengzhou 450046, China.

出版信息

Int J Mol Sci. 2024 Sep 25;25(19):10316. doi: 10.3390/ijms251910316.

DOI:10.3390/ijms251910316
PMID:39408647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11477197/
Abstract

The liver plays an important role in regulating lipid metabolism in animals. This study investigated the function and mechanism of in liver lipid metabolism in hens at the peak of egg production. The effect of on intracellular lipid content in LMH cells was evaluated by qPCR, Oil Red O staining, and detection of triglyceride (TG) and cholesterol (TC) content. The interaction between and MYH9 was confirmed by RNA purification chromatin fractionation (CHIRP) and RNA immunoprecipitation (RIP) analysis. The results showed that increased the intracellular content of TG and TC and promoted the expression of genes related to lipid synthesis. It was further found that had a negative regulatory effect on the expression level of MYH9 protein in LMH cells. The intracellular TG and TC content of MYH9 knockdown cells increased, and the expression of genes related to lipid decomposition was significantly reduced. In addition, this study confirmed that the role of is at least partly through mediating the ubiquitination of MYH9 protein to accelerate the degradation of MYH9 protein. This discovery provides a new molecular target for improving egg-laying performance in hens and treating fatty liver disease in humans.

摘要

肝脏在动物的脂质代谢调节中起着重要作用。本研究探讨了 在产蛋高峰期母鸡肝脏脂质代谢中的功能和机制。通过 qPCR、油红 O 染色以及甘油三酯 (TG) 和胆固醇 (TC) 含量的检测，评估了 在 LMH 细胞内的脂质含量的影响。通过 RNA 纯化染色质分馏 (CHIRP) 和 RNA 免疫沉淀 (RIP) 分析证实了 与 MYH9 之间的相互作用。结果表明， 增加了 TG 和 TC 的细胞内含量，并促进了与脂质合成相关的基因的表达。进一步发现， 在 LMH 细胞中对 MYH9 蛋白表达水平具有负调控作用。MYH9 敲低细胞的细胞内 TG 和 TC 含量增加，与脂质分解相关的基因表达明显降低。此外，本研究证实 的作用至少部分是通过介导 MYH9 蛋白的泛素化来加速 MYH9 蛋白的降解。这一发现为提高母鸡产蛋性能和治疗人类脂肪肝疾病提供了新的分子靶标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/11477197/7d8dde0803ec/ijms-25-10316-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/11477197/3d81667617c0/ijms-25-10316-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/798e/11477197/df2ba67428ff/ijms-25-10316-g003.jpg
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