Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Int J Mol Sci. 2024 Sep 26;25(19):10364. doi: 10.3390/ijms251910364.
The Chinese rare minnow (), an important model fish in China, faces endangerment in the wild. Sperm cryopreservation facilitates the development of new strains and germplasm conservation, but the quality of its cryopreserved sperm remains low. This study evaluates the protective effects of different concentrations of antifreeze proteins (AFP I and AFP III) on the cryopreservation of Chinese rare minnow sperm. Cryopreserved sperm showed significant declines in progressive motility, curvilinear velocity (VCL), average path velocity (VAP), and lifespan compared to fresh sperm, except for straight-line velocity (VSL). The cryomedium containing 10 μg/mL AFP I improved these parameters to their highest levels. However, no significant difference was found in progressive motility and kinetic parameters between cryopreserved sperm with and without AFPs. Cryopreserved sperm with 10 μg/mL AFP I showed the highest plasma membrane integrity, mitochondrial activity, and DNA integrity, significantly better than without AFPs; importantly, the fertilization rate of cryopreserved sperm with 10 μg/mL AFP I was not significantly different from that of fresh sperm. These results indicate that the addition of 10 μg/mL AFP I to the cryomedium for Chinese rare minnow sperm does not improve kinetic parameters but significantly enhances sperm quality, aiding in its new strain development and germplasm conservation.
中国稀有鮈鲫（），中国重要的鱼类模式生物，在野外面临濒危。精子冷冻保存有利于新株系的开发和种质资源的保存，但冷冻保存精子的质量仍然较低。本研究评估了不同浓度的抗冻蛋白（AFP I 和 AFP III）对中国稀有鮈鲫精子冷冻保存的保护作用。与新鲜精子相比，冷冻保存的精子在直线速度（VSL）以外的各项运动参数，如直线运动速度（VSL）、曲线运动速度（VCL）、平均路径速度（VAP）和生存时间，均显著下降。含 10μg/mL AFP I 的冷冻保护液可将这些参数提高到最高水平。然而，添加和不添加 AFP 的冷冻保存精子的运动参数和动力学参数没有显著差异。含 10μg/mL AFP I 的冷冻保存精子的质膜完整性、线粒体活性和 DNA 完整性最高，明显优于不添加 AFP 的精子；重要的是，含 10μg/mL AFP I 的冷冻保存精子的受精率与新鲜精子没有显著差异。这些结果表明，在冷冻保护液中添加 10μg/mL AFP I 虽然不能提高精子的运动参数，但能显著提高精子的质量，有助于新株系的开发和种质资源的保存。
