The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions, Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.
Int J Mol Sci. 2024 Sep 30;25(19):10574. doi: 10.3390/ijms251910574.
Laccase, a copper-containing oxidoreductase, has close links with secondary metabolite biosynthesis in plants. Its activity can affect the synthesis and accumulation of secondary metabolites, thereby influencing plant growth, development, and stress resistance. This study aims to identify the grape laccases () gene family members in grape ( L.) and explore the transcriptional regulatory network in berry development. Here, 115 were identified and divided into seven (Type I-VII) classes. These were distributed on 17 chromosomes and out of 47 on chromosome 18, 34 (72.34%) were involved in tandem duplication events. , , , and were highly expressed before fruit color development, while , , , , , , and were highly expressed after fruit color transformation. Notably, showed a significant positive correlation with important metabolites including resveratrol, resveratrol dimer, and peonidin-3-glucoside. Analysis of the transcriptional regulatory network predicted that the 12 different transcription factors target s genes. Specifically, WRKY and ERF were identified as potential transcriptional regulatory factors for , while Dof and MYB were identified as potential transcriptional regulatory factors for . This study identifies and provides basic information on the grape LAC gene family members and, in combination with transcriptome and metabolome data, predicts the upstream transcriptional regulatory network of s.
漆酶是一种含铜的氧化还原酶，与植物次生代谢物的生物合成密切相关。其活性可以影响次生代谢物的合成和积累，从而影响植物的生长、发育和抗逆性。本研究旨在鉴定葡萄（ Vitis vinifera ）中的漆酶（ ）基因家族成员，并探讨其在浆果发育过程中的转录调控网络。本研究共鉴定出 115 个 ，并将其分为 7 个（I-VII 型）。这些基因分布在 17 条染色体上，其中 18 号染色体上有 47 个，34 个（72.34%）为串联重复事件。在果实颜色发育前， 、 、 、 和 高度表达，而在果实颜色转变后， 、 、 、 、 、 和 高度表达。值得注意的是， 与包括白藜芦醇、白藜芦醇二聚体和矢车菊素-3-葡萄糖苷在内的重要代谢物呈显著正相关。转录调控网络分析预测，12 种不同的转录因子靶向 s 基因。具体来说，WRKY 和 ERF 被鉴定为 基因的潜在转录调控因子，而 Dof 和 MYB 被鉴定为 基因的潜在转录调控因子。本研究鉴定并提供了葡萄 LAC 基因家族成员的基本信息，并结合转录组和代谢组数据，预测了 s 的上游转录调控网络。
