Hasegawa M, Usui H, Araki K, Kuwano R, Takahashi Y
FEBS Lett. 1986 Jan 6;194(2):224-6. doi: 10.1016/0014-5793(86)80089-8.
Since the nucleotide sequence of cholecystokinin (CCK) cDNA was found in the rat gene, we applied cDNA to quantitate the CCK mRNA. The size of the mRNA for a CCK precursor was 850 nucleotides in length using brain cytoplasmic RNA. There were no bands except CCK mRNA by Northern blot analysis. We also examined the developmental changes and regional distribution of CCK mRNA in rat brains by dot-blot and gel-blot hybridization using CCK cDNA as a probe. CCK mRNA was barely detectable in the fetal brain, but started to increase postnatally and attained the plateau level after 20-30 days. Further, the level of CCK mRNA was highest in the frontal cortex, followed by those of the hippocampus and striatum. The cerebellum contained only negligible CCK mRNA. These results are in agreement with those of CCK concentration in the corresponding brain areas and suggest a transcriptional control of CCK concentration.
自从在大鼠基因中发现胆囊收缩素（CCK）cDNA的核苷酸序列后，我们应用cDNA来定量CCK mRNA。使用脑细胞质RNA时，CCK前体mRNA的大小为850个核苷酸。通过Northern印迹分析，除了CCK mRNA外没有其他条带。我们还使用CCK cDNA作为探针，通过斑点印迹和凝胶印迹杂交研究了大鼠脑中CCK mRNA的发育变化和区域分布。在胎儿脑中几乎检测不到CCK mRNA，但出生后开始增加，并在20 - 30天后达到平台水平。此外，额叶皮质中CCK mRNA的水平最高，其次是海马体和纹状体。小脑中仅含有可忽略不计的CCK mRNA。这些结果与相应脑区中CCK浓度的结果一致，并提示了CCK浓度的转录调控。
