StEPF2 和 StEPFL9 在调控马铃薯气孔发育和耐旱性方面发挥着相反的作用。

StEPF2 and StEPFL9 Play Opposing Roles in Regulating Stomatal Development and Drought Tolerance in Potato ( L.).

机构信息

Key Laboratory of Nanchong City of Ecological Environment Protection and Pollution Prevention in Jialing River Basin, College of Environmental Science and Engineering, China West Normal University, Nanchong 637002, China.

Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.

出版信息

Int J Mol Sci. 2024 Oct 5;25(19):10738. doi: 10.3390/ijms251910738.

DOI:10.3390/ijms251910738

PMID:39409067

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11476617/

Abstract

Stomata are essential for photosynthesis and water-use efficiency in plants. When expressed in transgenic plants, the potato () proteins EPIDERMAL PATTERNING FACTOR 2 (StEPF2) and StEPF-LIKE9 (StEPFL9) play antagonistic roles in regulating stomatal density. Little is known, however, about how these proteins regulate stomatal development, growth, and response to water deficit in potato. Transgenic potato plants overexpressing (E2 plants) or (ST plants) were generated, and RT-PCR and Western blot analyses were used to select two lines overexpressing each gene. E2 plants showed reduced stomatal density, whereas ST plants produced excessive stomata. Under well-watered conditions, ST plants displayed vigorous growth with improved leaf gas exchange and also showed increased biomass/yields compared with non-transgenic and E2 plants. E2 plants maintained lower HO content and higher levels of stomatal conductance and photosynthetic capacity than non-transgenic and ST plants, which resulted in higher water-use efficiency and biomass/yields during water restriction. These results suggest that StEPF2 and StEPFL9 functioned in pathways regulating stomatal development. These genes are thus promising candidates for use in future breeding programs aimed at increasing potato water-use efficiency and yield under climate change scenarios.

摘要

气孔对于植物的光合作用和水分利用效率至关重要。在转基因植物中表达时，马铃薯（）蛋白表皮图案形成因子 2（StEPF2）和 StEPF-LIKE9（StEPFL9）在调节气孔密度方面发挥拮抗作用。然而，关于这些蛋白如何调节马铃薯气孔发育、生长以及对水分亏缺的反应，人们知之甚少。生成了过表达（E2 植物）或（ST 植物）的转基因马铃薯植物，并进行 RT-PCR 和 Western blot 分析以选择过表达每种基因的两条系。E2 植物表现出气孔密度降低，而 ST 植物产生过多的气孔。在充分浇水的条件下，ST 植物表现出旺盛的生长，叶片气体交换得到改善，与非转基因和 E2 植物相比，生物量/产量也有所增加。E2 植物比非转基因和 ST 植物保持更低的 HO 含量和更高的气孔导度和光合能力，这导致在水分限制期间具有更高的水分利用效率和生物量/产量。这些结果表明，StEPF2 和 StEPFL9 在调节气孔发育的途径中起作用。因此，这些基因是未来在气候变化情景下提高马铃薯水分利用效率和产量的育种计划中的有前途的候选基因。