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牡丹胚胎发生愈伤组织形成过程中与PoSERK相互作用蛋白PorbcL的功能分析（T. Hong和J. X. Zhang）

Functional Analysis of the PoSERK-Interacting Protein PorbcL in the Embryogenic Callus Formation of Tree Peony ( T. Hong et J. X. Zhang).

作者信息

Song Yinglong, Wang Jiange, Zhu Jiale, Shang Wenqian, Jia Wenqing, Sun Yuke, He Songlin, Yang Xitian, Wang Zheng

机构信息

Postdoctoral Innovation Practice Base, Henan Institute of Science and Technology, Xinxiang 453003, China.

Postdoctoral Workstation, Henan Bainong Seed Industry Co., Ltd., Xinxiang 453003, China.

出版信息

Plants (Basel). 2024 Sep 26;13(19):2697. doi: 10.3390/plants13192697.

DOI:10.3390/plants13192697
PMID:39409567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11479246/
Abstract

is a marker gene for early somatic embryogenesis. We screened and functionally verified a SERK-interacting protein to gain insights into tree-peony somatic embryogenesis. Using PoSERK as bait, we identified PorbcL (i.e., the large subunit of Rubisco) as a SERK-interacting protein from a yeast two-hybrid (Y2H) library of cDNA from developing tree-peony somatic embryos. The interaction between PorbcL and PoSERK was verified by Y2H and bimolecular fluorescence complementation analyses. encodes a 586-amino-acid acidic non-secreted hydrophobic non-transmembrane protein that is mainly localized in the chloroplast and plasma membrane. was highly expressed in tree-peony roots and flowers and was up-regulated during zygotic embryo development. overexpression caused the up-regulation of (encoding somatic embryogenesis receptor-like kinase), (encoding agamous-like 15), and (encoding glucose-6-phosphate translocator), while it caused the down-regulation of (encoding leafy cotyledon 1) in tree-peony callus. overexpression led to increased indole-3-acetic acid (IAA) content but decreasing contents of abscisic acid (ABA) and 6-benzyladenosine (BAPR). The changes in gene expression, high IAA levels, and increased ratio of IAA to ABA, BAPR, 1-Aminocyclopropanecarboxylic acid (ACC), 5-Deoxystrigol (5DS), and brassinolide (BL) promoted embryogenesis. These results provide a foundation for establishing a tree-peony embryogenic callus system.

摘要

是早期体细胞胚胎发生的标记基因。我们筛选并对一种SERK相互作用蛋白进行了功能验证，以深入了解牡丹体细胞胚胎发生。以PoSERK为诱饵，我们从发育中的牡丹体细胞胚胎的cDNA酵母双杂交（Y2H）文库中鉴定出PorbcL（即核酮糖-1,5-二磷酸羧化酶/加氧酶的大亚基）作为一种SERK相互作用蛋白。通过Y2H和双分子荧光互补分析验证了PorbcL与PoSERK之间的相互作用。 编码一种586个氨基酸的酸性非分泌型疏水非跨膜蛋白，主要定位于叶绿体和质膜。 在牡丹根和花中高度表达，在合子胚发育过程中上调。 在牡丹愈伤组织中过表达导致 （编码体细胞胚胎发生受体样激酶）、 （编码类AGAMOUS 15）和 （编码葡萄糖-6-磷酸转运体）上调，而导致 （编码叶状子叶1）下调。 过表达导致吲哚-3-乙酸（IAA）含量增加，但脱落酸（ABA）和6-苄基腺嘌呤（BAPR）含量降低。基因表达的变化、高IAA水平以及IAA与ABA、BAPR、1-氨基环丙烷-1-羧酸（ACC）、5-脱氧独脚金醇（5DS）和油菜素内酯（BL）比例的增加促进了胚胎发生。这些结果为建立牡丹胚性愈伤组织系统奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4961/11479246/1dfcbc7c29eb/plants-13-02697-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4961/11479246/c934cb5d712f/plants-13-02697-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4961/11479246/c0f31a0da6e9/plants-13-02697-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4961/11479246/ad38532265c8/plants-13-02697-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4961/11479246/fdbd23888d24/plants-13-02697-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4961/11479246/3a8b02817a9a/plants-13-02697-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4961/11479246/bfcb9052f9b4/plants-13-02697-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4961/11479246/1dfcbc7c29eb/plants-13-02697-g011.jpg

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