Zhang Yihua, Lu Xing, Yao Wenrong, Cheng Xiaoqing, Wang Qiao, Feng Yu, Shen Wenbiao
College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China.
College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Plants (Basel). 2024 Oct 8;13(19):2819. doi: 10.3390/plants13192819.
Despite substantial evidence suggesting that hydrogen gas (H) can enhance osmotic tolerance in plants, the conventional supply method of hydrogen-rich water (HRW) poses challenges for large-scale agricultural applications. Recently, magnesium hydride (MgH), a hydrogen storage material in industry, has been reported to yield beneficial effects in plants. This study aimed to investigate the effects and underlying mechanisms of MgH in plants under osmotic stress. Mung bean seedlings were cultured under control conditions or with 20% polyethylene glycol (PEG)-6000, with or without MgH addition (0.01 g L). Under our experimental conditions, the MgH solution maintained a higher H content and longer retention time than HRW. Importantly, PEG-stimulated endogenous H production was further triggered by MgH application. Further results revealed that MgH significantly alleviated the inhibition of seedling growth and reduced oxidative damage induced by osmotic stress. Pharmacological evidence suggests the MgH-reestablished redox homeostasis was associated with activated antioxidant systems, particularly the ascorbate-glutathione cycle. The above observations were further supported by the enhanced activities and gene transcriptional levels of ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, and glutathione reductase. Overall, this study demonstrates the importance of MgH in mitigating osmotic stress in mung bean seedlings, providing novel insights into the potential agricultural applications of hydrogen storage materials.
尽管有大量证据表明氢气（H₂）可以增强植物的渗透耐受性，但富氢水（HRW）的传统供应方法给大规模农业应用带来了挑战。最近，氢化镁（MgH₂）作为一种工业储氢材料，已被报道对植物有有益影响。本研究旨在探讨MgH₂在渗透胁迫下对植物的影响及其潜在机制。绿豆幼苗在对照条件下或添加20%聚乙二醇（PEG）-6000的条件下培养，添加或不添加MgH₂（0.01 g/L）。在我们的实验条件下，MgH₂溶液比HRW保持更高的H₂含量和更长的保留时间。重要的是，MgH₂的施用进一步触发了PEG刺激的内源性H₂产生。进一步的结果表明，MgH₂显著减轻了渗透胁迫对幼苗生长的抑制并减少了氧化损伤。药理学证据表明，MgH₂重建的氧化还原稳态与激活的抗氧化系统有关，特别是抗坏血酸-谷胱甘肽循环。抗坏血酸过氧化物酶、单脱氢抗坏血酸还原酶、脱氢抗坏血酸还原酶和谷胱甘肽还原酶的活性增强和基因转录水平提高进一步支持了上述观察结果。总体而言，本研究证明了MgH₂在减轻绿豆幼苗渗透胁迫方面的重要性，并为储氢材料在农业上的潜在应用提供了新的见解。
