Shiraya K, Nagataki S
Invest Ophthalmol Vis Sci. 1986 Jan;27(1):24-8.
The movement of fluorescein monoglucuronide, a fluorescent metabolite of fluorescein, was studied in the rabbit cornea in vitro and in vivo. A stromal strip was exposed to fluorescein monoglucuronide, and the diffusion rate and the distribution in the stroma were measured every hr for 24 hr. The diffusion coefficient was 0.94 +/- 0.11 (+/- S.D.) X 10(-6) cm2/sec, and the saline/stroma distribution ratio was in a range of 0.67 to 0.69. The concentration of fluorescein monoglucuronide in the anterior chamber and the cornea was measured every hr for 8 hr following intravenous administration. The endothelial permeability was 4.7 +/- 1.0 X 10(-4) cm/min, and the aqueous/cornea distribution ratio was 0.56 +/- 0.05. It appears that the corneal endothelial permeability in the living eye determined hitherto from systemic administration of fluorescein is most likely the permeability to fluorescein monoglucuronide.
在体外和体内的兔角膜中研究了荧光素的荧光代谢产物单葡糖醛酸荧光素的移动情况。将一块角膜基质条暴露于单葡糖醛酸荧光素中，在24小时内每小时测量其在基质中的扩散速率和分布情况。扩散系数为0.94±0.11（±标准差）×10⁻⁶平方厘米/秒，盐水/基质分布比在0.67至0.69范围内。静脉给药后8小时内每小时测量前房和角膜中单葡糖醛酸荧光素的浓度。内皮通透性为4.7±1.0×10⁻⁴厘米/分钟，房水/角膜分布比为0.56±0.05。迄今为止，通过全身给予荧光素所测定的活体眼中角膜内皮通透性很可能是对单葡糖醛酸荧光素的通透性。
