Pasaoglu Bozkurt Aylin, Demirci Mehmet, Erdogan Pelin, Kayalar Emre
Department of Orthodontics, Faculty of Dentistry, Istanbul Aydin University, Istanbul, Turkey.
Department of Medical Microbiology, Kirklareli University, Faculty of Medicine, Kirklareli, Turkey.
Am J Orthod Dentofacial Orthop. 2025 Jan;167(1):47-62. doi: 10.1016/j.ajodo.2024.08.010. Epub 2024 Oct 16.
This study aimed to compare and evaluate time-dependent biofilm formation and microbial adhesion on 6 different clear aligner systems: Invisalign (Align Technology, San Jose, Calif), Clarity (3M ESPE Maplewood, Minn), ClearCorrect (Institut Straumann AG, Basel, Switzerland), Smartee (Smartee Denti-Technology, Shanghai, China), Orthero (Orthero, Istanbul, Turkey) and Graphy (Graphy Inc, Seoul, South Korea).
Streptococcus mutans (ATCC 25175) and Lactobacillus acidophilus (ATCC 4356) were used to evaluate the microbial adhesion and biofilm formation on orthodontic clear aligners at the 0, 24, 48, 72, 96, 120, 168, and 240 hours. Two-way repeated measures analysis of variance (Greenhouse-Geisser) test and post-hoc Bonferroni T2 tests were used for statistical evaluation. The statistical significance level was set at P <0.05.
It was found that more bacterial formation occurred on ClearCorrect than on Smartee at 120, 168, and 240 hours (P <0.05). It was observed more biofilm formation at 168 hours on Graphy than on Smartee (P <0.05). It was found that S mutans + L acidophilus formed more biofilm at 120 and 168 hours on Graphy than on Invisalign (P <0.05).
Elevated biofilm formation across all materials carries substantial clinical implications. Orthodontists and patients should remain aware of the increased risk of microbial colonization with extended aligner usage.
本研究旨在比较和评估6种不同的透明矫治器系统随时间变化的生物膜形成情况和微生物黏附情况，这6种系统分别是：隐适美（爱齐科技公司，加利福尼亚州圣何塞）、Clarity（3M ESPE公司，明尼苏达州枫木伍德）、ClearCorrect（士卓曼集团，瑞士巴塞尔）、时代天使（时代天使牙科科技，中国上海）、奥美科（奥美科，土耳其伊斯坦布尔）和速达齐（速达齐公司，韩国首尔）。
使用变形链球菌（ATCC 25175）和嗜酸乳杆菌（ATCC 4356）评估正畸透明矫治器在0、24、48、72、96、120、168和240小时时的微生物黏附及生物膜形成情况。采用双向重复测量方差分析（Greenhouse-Geisser）检验和事后Bonferroni T2检验进行统计学评估。统计学显著性水平设定为P<0.05。
发现在120、168和240小时时，ClearCorrect上形成的细菌比时代天使上更多（P<0.05）。观察到在168小时时，速达齐上形成的生物膜比时代天使上更多（P<0.05）。发现在120和168小时时，变形链球菌+嗜酸乳杆菌在速达齐上形成的生物膜比在隐适美上更多（P<0.05）。
所有材料上生物膜形成增加具有重大临床意义。正畸医生和患者应意识到随着矫治器使用时间延长，微生物定植风险增加。
