Krishnendu M R, Bhagat Stuti, Jain Vidhi, Mehta Divya, Singh Sanjay
Nanobiology and Nanozymology Laboratory, National Institute of Animal Biotechnology (NIAB), Opposite Journalist Colony, Near Gowlidoddy, Extended Q-City Road, Gachibowli, Hyderabad, Telangana 500032, India.
Nanobiology and Nanozymology Laboratory, National Institute of Animal Biotechnology (NIAB), Opposite Journalist Colony, Near Gowlidoddy, Extended Q-City Road, Gachibowli, Hyderabad, Telangana 500032, India; Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University, Central Campus, Navrangpura, Ahmedabad, Gujarat 380009, India.
Colloids Surf B Biointerfaces. 2025 Jan;245:114303. doi: 10.1016/j.colsurfb.2024.114303. Epub 2024 Oct 9.
Minimally invasive methods for detecting glucose, cholesterol and hydrogen peroxide are crucial for monitoring the nutritional and health status of humans and animals. The peroxidase mimetic activity by nanozymes is one of the versatile methods for detecting glucose, cholesterol, hydrogen peroxide, and other biomolecules. However, the strict requirement of acidic pH limits their sensing and interfacing ability with natural enzymes. The present study developed bovine serum albumin (BSA) coated gold nanoclusters (AuNC) immobilized on paper fabric to enable single-step visual detection of glucose, cholesterol and hydrogen peroxide in complex biological fluids like serum and milk. The BSA-AuNC suspension and immobilized paper fabric synergistically interface with the natural oxidative enzymes, glucose oxidase or cholesterol oxidase, at physiological pH. The concomitant loss in the fluorescent intensity of BSA-AuNC-loaded paper fabric exposed to the generated hydrogen peroxide (glucose/glucose oxidase or cholesterol/cholesterol oxidase) was directly proportional to the concentration of glucose or cholesterol. These reactions enabled simple visual detection as well as quantification of hydrogen peroxide, glucose and cholesterol using Image-J software and common smartphone-based mobile applications. The detection ability of BSA-AuNC-embedded paper fabric is specific and remains unaltered in the presence of similar oxidase enzymes or similar substrate analogues. With these unique features, the BSA-AuNC embedded paper fabric stands out as a prominent analytical device with enormous potential as a simple, user-friendly detection tool for monitoring biomolecules that are important to health, nutrition, and environmental safeguarding.
检测葡萄糖、胆固醇和过氧化氢的微创方法对于监测人类和动物的营养与健康状况至关重要。纳米酶的过氧化物酶模拟活性是检测葡萄糖、胆固醇、过氧化氢及其他生物分子的通用方法之一。然而，对酸性pH的严格要求限制了它们与天然酶的传感和接口能力。本研究开发了固定在纸质织物上的牛血清白蛋白（BSA）包被金纳米簇（AuNC），以实现对血清和牛奶等复杂生物流体中葡萄糖、胆固醇和过氧化氢的单步可视化检测。在生理pH值下，BSA-AuNC悬浮液和固定化纸质织物与天然氧化酶（葡萄糖氧化酶或胆固醇氧化酶）协同作用。暴露于生成的过氧化氢（葡萄糖/葡萄糖氧化酶或胆固醇/胆固醇氧化酶）时，负载BSA-AuNC的纸质织物荧光强度的相应损失与葡萄糖或胆固醇的浓度成正比。这些反应实现了简单的可视化检测，以及使用Image-J软件和基于智能手机的普通移动应用程序对过氧化氢、葡萄糖和胆固醇进行定量。嵌入BSA-AuNC的纸质织物的检测能力具有特异性，在存在类似氧化酶或类似底物类似物的情况下保持不变。凭借这些独特特性，嵌入BSA-AuNC的纸质织物作为一种杰出的分析设备脱颖而出，作为一种简单、用户友好的检测工具，在监测对健康、营养和环境保护至关重要的生物分子方面具有巨大潜力。
