利用二维银基底增强对结直肠癌生物标志物的表面增强拉曼散射检测。

Enhanced SERS detection of the colorectal cancer biomarker utilizing a two-dimensional silver substrate.

作者信息

Nie Qingling, Xu Peipei, Liu Jiajia, Wang Yanhanzhuo, Huang Qian, You Ruiyun, Liu Yunzhen, Li Lizhi, Lu Yudong

机构信息

College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Engineering Research Center of Industrial Biocatalysts, Fujian Province Higher Education Institutes, Fujian Normal University, Fuzhou, Fujian 350007, China.

出版信息

Colloids Surf B Biointerfaces. 2025 Jan;245:114317. doi: 10.1016/j.colsurfb.2024.114317. Epub 2024 Oct 14.

DOI:10.1016/j.colsurfb.2024.114317

PMID:39413486

Abstract

To improve the sensitivity, accuracy and specificity of the assay, a two-dimensional silver substrate with EF=5.85×10 was first synthesized as a SERS substrate, on the surface of which DSP molecules were modified to form a DSP-antibody coupling through the activation of two N-hydroxy succinimide (NHS) esters to capture TNF-α. Subsequently, aptamerized silver-coated gold nanospheres (Au@TFMBA@Ag) were synthesized as Surface-Enhanced Raman Scattering (SERS) recognition probes. These probes were employed to create a sandwich structure for the quantitative detection of Tumor Necrosis Factor-alpha (TNF-α), utilizing the SERS signal intensity at 1374 cm. Quantitative detection of TNF-α was successfully accomplished within the concentration range of 10 to 10 mg·mL. Clinical serum samples were collected and subjected to testing. Significance analysis, conducted through the T-test (p < 0.0001), unequivocally showed the method's ability to differentiate between sera from normal individuals and those diagnosed with colon cancer.

摘要

为提高检测的灵敏度、准确性和特异性，首先合成了一种二维银基底（EF = 5.85×10）作为表面增强拉曼散射（SERS）基底，在其表面修饰DSP分子，通过激活两个N-羟基琥珀酰亚胺（NHS）酯形成DSP-抗体偶联物以捕获肿瘤坏死因子-α（TNF-α）。随后，合成了适配体化的银包金纳米球（Au@TFMBA@Ag）作为表面增强拉曼散射（SERS）识别探针。利用这些探针构建夹心结构，通过1374 cm处的SERS信号强度对肿瘤坏死因子-α（TNF-α）进行定量检测。在10至10 mg·mL的浓度范围内成功完成了TNF-α的定量检测。收集临床血清样本并进行检测。通过T检验（p < 0.0001）进行的显著性分析明确表明该方法能够区分正常个体和结肠癌患者的血清。