Thu Dau Mot University, Department of Medicine and Pharmacy, Thu Dau Mot, Binh Duong, Vietnam.
Vietnam National University, VNU University of Science, Department of Biology and Biotechnology, Ho Chi Minh City, Vietnam.
Braz J Biol. 2024 Oct 14;84:e287203. doi: 10.1590/1519-6984.287203. eCollection 2024.
This study aimed to investigate the impact of Vernonia amygdalina leaf extract on FLT3 regulation. V. amygdalina was extracted with 96% ethanol (VAE-96), and its cytotoxicity against FLT3- cell lines (MOLM-13 and MV-4-11) was evaluated. The results indicated that VAE-96 induced apoptosis in these cells and inhibited the phosphorylation of AKT, MAPK, and FLT3. Additionally, VAE-96 substantially diminished the activity of the FLT3 promoter and the expression of FLT3 mRNA. The extract was found to contain alkaloids, saponin, reduced sugar compounds, and polyphenols, including tannins and flavonoids, as per the phytoconstituents analysis. The potential of alkaloid fractions on MOLM-13 cells was indicated by the robust cytotoxic effect of the alkaloid fractions, which resulted in over 50% cell mortality at 30 µg/ml. Our results suggest that VAE-96 may be a beneficial agent for the prevention and treatment of AML with FLT3-ITD mutation.
本研究旨在探讨辣木叶提取物对 FLT3 调节的影响。采用 96%乙醇提取辣木叶（VAE-96），并评估其对 FLT3-细胞系（MOLM-13 和 MV-4-11）的细胞毒性。结果表明，VAE-96 诱导这些细胞凋亡，并抑制 AKT、MAPK 和 FLT3 的磷酸化。此外，VAE-96 显著降低了 FLT3 启动子的活性和 FLT3 mRNA 的表达。根据植物成分分析，提取物中含有生物碱、皂苷、还原糖化合物和多酚，包括单宁和类黄酮。生物碱部分对 MOLM-13 细胞的潜在作用表明，生物碱部分具有很强的细胞毒性作用，在 30µg/ml 时导致超过 50%的细胞死亡率。我们的结果表明，VAE-96 可能是预防和治疗具有 FLT3-ITD 突变的 AML 的有益药物。
