Albina J E, Shearer J, Mastrofrancesco B, Caldwell M D
Am J Physiol. 1986 Jan;250(1 Pt 1):E24-30. doi: 10.1152/ajpendo.1986.250.1.E24.
To evaluate whether the alterations in amino acid (AA) metabolism that follow the lambda-carrageenan injury are direct consequences of wounding and are therefore independent of food intake, plasma AA, in vivo muscle intracellular free AA, and AA release during isolated hindlimb perfusions were determined in pentobarbital-anesthetized rats with lambda-carrageenan-induced hindlimb muscle wounds (W) and in pair-fed (PFC) and ad libitum-fed (ALC) non-wounded controls. Both PFC and W animals showed different plasma and muscle intracellular AA composition that ALC. The alterations observed in these compartments in W animals may not have resulted exclusively as a consequence of the wound. Wounded hindlimbs released more AA during perfusion than either control group. The marked increase in net protein catabolism of W muscle appeared to be related to wounding and not a consequence of partial starvation. The normalized release (amino acid/phenylalanine ratio) of glutamine (W less than ALC), alanine (W less than PFC and ALC), and the branched-chain amino acids (W greater than PFC and ALC) differed among groups, suggesting an alteration in the metabolism of these AA in W tissue.
为评估角叉菜胶损伤后氨基酸（AA）代谢的改变是否是创伤的直接后果，因而与食物摄入无关，我们测定了戊巴比妥麻醉的、有角叉菜胶诱导后肢肌肉创伤（W）的大鼠以及配对喂养（PFC）和自由进食（ALC）的非创伤对照大鼠的血浆AA、体内肌肉细胞内游离AA以及离体后肢灌注期间的AA释放。PFC组和W组动物的血浆和肌肉细胞内AA组成均与ALC组不同。在W组动物这些区室中观察到的改变可能并非完全由创伤导致。创伤后肢在灌注期间释放的AA比任一对照组都多。W组肌肉净蛋白分解代谢的显著增加似乎与创伤有关，而非部分饥饿的结果。各组之间谷氨酰胺（W组＜ALC组）、丙氨酸（W组＜PFC组和ALC组）以及支链氨基酸（W组＞PFC组和ALC组）的标准化释放（氨基酸/苯丙氨酸比率）有所不同，表明W组组织中这些AA的代谢发生了改变。
