Morrissey J, Windus D, Schwab S, Tannenbaum J, Klahr S
Am J Physiol. 1986 Jan;250(1 Pt 2):F136-43. doi: 10.1152/ajprenal.1986.250.1.F136.
Unilateral ureteral obstruction of 24 h duration in the dog results in a 20% decrease in the amount of total phospholipids present in basolateral membranes of renal tubular cells obtained from the experimental kidney as compared with the amount of phospholipid in basolateral membranes prepared from the contralateral kidney of the same dogs or from the kidneys of normal sham-operated dogs. There was also a decrease in the content of cholesterol and cholesterol esters of the basolateral membranes from the experimental kidney. By contrast, no significant change in lipid content was observed in brush border membranes obtained from the experimental kidney of dogs with unilateral ureteral obstruction. The decrease in phospholipid content of basolateral membranes was accompanied by a 40% fall in the content of phosphatidylcholine and a 12% fall in sphingomyelin. There was a small (12%) but significant increase in the content of phosphatidylethanolamine in basolateral membranes. The mechanisms responsible for the selective decrease in phospholipid content of basolateral membranes remain to be established. It is postulated that changes in solute transport and altered response to hormones observed in the postobstructed kidney of animals with unilateral ureteral obstruction may be explained, at least in part, by changes in the lipid composition of basolateral membranes.
狗单侧输尿管梗阻24小时后，与取自同一只狗对侧肾脏或正常假手术狗肾脏的肾小管细胞基底外侧膜中的磷脂量相比，实验侧肾脏肾小管细胞基底外侧膜中的总磷脂量减少了20%。实验侧肾脏基底外侧膜中的胆固醇和胆固醇酯含量也有所下降。相比之下，单侧输尿管梗阻狗的实验侧肾脏刷状缘膜中的脂质含量未观察到显著变化。基底外侧膜磷脂含量的减少伴随着磷脂酰胆碱含量下降40%和鞘磷脂含量下降12%。基底外侧膜中磷脂酰乙醇胺的含量有小幅（12%）但显著的增加。基底外侧膜磷脂含量选择性降低的机制尚待确定。据推测，在单侧输尿管梗阻动物的梗阻后肾脏中观察到的溶质转运变化和对激素反应的改变，至少部分可以由基底外侧膜脂质组成的变化来解释。
