Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran AND Department of Biology, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran.
Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Allergy Asthma Immunol. 2024 May 27;23(3):272-287. doi: 10.18502/ijaai.v23i3.15637.
Reactivation of Polyomavirus BK (BKPyV) is related to reduction of T cells response in kidney transplant recipients (KTRs). Here, we examined the differentiation of CD4+ T cells subsets in response to BKPyV KTRs, using the BKPyV VP1 (viral capsid protein 1) as a stimulator. We categorized our samples into three distinct groups: 1. Reactive BKPyV (BKPyV+), 2. non-reactive (BKPyV-) KTRs and 3. Healthy controls. BKPyV- KTRs and healthy controls stimulated with VP1 and BKPyV+ unstimulated with VP1. The human CD4+ T cells was stimulation with VP1-Ag. The proportion of CD4+ T lymphocytes and their various subsets, including naive T cells, central memory T cells (TCM), and effector memory T cells (TEM) was measured using flowcytometry. BKPyV- KTRs VP1+ indicated significantly lower TCM CD4+ T cells in contrast with both BKPyV+ KTRs VP1-, and healthy controls VP1+. This indicates that VP1 stimulation may reduce TCM cell levels in these patients. The percentage of TEM in the BKPyV- KTRs VP1+ group was significantly less prevalent than the BKPyV+ KTRs VP1- group. The percentage of TEM cells in BKPyV+ KTRs VP1- was significantly lower than the healthy controls VP1+. Stimulation with VP1 protein significantly increased the frequency of cytotoxic CD4+ T cells in BKPyV- KTRs VP1+ compared to BKPyV+ KTRs VP1-. The present research has shown that the VP1 stimulation of CD4+ T cells can induce cytotoxic CD4+ T cells responses that may help overcome BKPyV infection in KTRs. However, VP1 stimulation may also differentially affect TCM and TEM CD4+ T cells subsets.
巨细胞病毒 BK（BKPyV）的再激活与肾移植受者（KTR）T 细胞反应的减少有关。在这里，我们使用 BKPyV VP1（病毒衣壳蛋白 1）作为刺激物，检查了对 BKPyV KTR 反应的 CD4+T 细胞亚群的分化。我们将我们的样本分为三组：1. 反应性 BKPyV（BKPyV+），2. 非反应性（BKPyV-）KTR 和 3. 健康对照。BKPyV- KTR 和健康对照用 VP1 刺激，BKPyV+未用 VP1 刺激。用 VP1-Ag 刺激人 CD4+T 细胞。使用流式细胞术测量 CD4+T 淋巴细胞及其各种亚群（包括幼稚 T 细胞、中央记忆 T 细胞（TCM）和效应记忆 T 细胞（TEM））的比例。与 BKPyV+ KTRs VP1-和健康对照 VP1+相比，BKPyV- KTRs VP1+的 TCM CD4+T 细胞明显较低。这表明 VP1 刺激可能会降低这些患者的 TCM 细胞水平。BKPyV- KTRs VP1+组的 TEM 百分比明显低于 BKPyV+ KTRs VP1-组。BKPyV+ KTRs VP1-的 TEM 细胞百分比明显低于健康对照 VP1+。VP1 蛋白刺激显著增加了与 BKPyV+ KTRs VP1-相比，BKPyV- KTRs VP1+的细胞毒性 CD4+T 细胞的频率。本研究表明，VP1 刺激 CD4+T 细胞可以诱导细胞毒性 CD4+T 细胞反应，这可能有助于克服 KTR 中的 BKPyV 感染。然而，VP1 刺激也可能对 TCM 和 TEM CD4+T 细胞亚群产生不同的影响。
