School of Pharmaceutical Sciences, Siksha 'O' Anusandhan Deemed to Be University, Bhubaneswar, 751003, Odisha, India.
School of Pharmacy and Life Sciences, Centurion University of Technology and Management, Bhubaneswar, 751050, India.
J Ethnopharmacol. 2025 Jan 30;337(Pt 3):118944. doi: 10.1016/j.jep.2024.118944. Epub 2024 Oct 16.
Tecoma stans (L.) Juss.ex Kunth (Bignoniaceae) is mainly found in tropical and subtropical regions of Africa and Asia. The leaves, flowers, roots, and bark are used to treat various aliments includes, skin infections, kidney problems, intestinal disorders, jaundice, toothaches, joint pain and repair cracked bones, antidotes for snake, scorpion, and rat bites.
The objective of the study is to assess the anti-arthritic properties of T. stans leaf using Complete Freund's adjuvant (CFA)-induced rat.
The ethanol extract of T. stans leaf (ETSL) was subjected toGas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for the identification of potential bioactive. The anti-arthritic activity was carried out by administering CFA (0.1 ml) into the sub-plantar surface of the right hind paw. The experimental animals were treated with indomethacin (10 mg/kg) and ETSL (250, 500 mg/kg) once a day orally for fourteen days. The arthritic parameters and hematological and biochemical parameters were evaluated using standard kit reagents. The levels of pro-inflammatory cytokines and inflammatory mediators were measured in blood serum. Antioxidant parameters were assessed in homogenized liver and joint tissues. Radiological and histopathological analysis of joint was performed. A computational molecular docking investigation of the phytoconstituents was conducted against COX-2, IL-1β, IL-6, and TNF-α receptors.
The ETSL at 500 mg/kg demonstrated significant (p < 0.01) restoration of arthritic parameters, hematological and biochemical indices and oxidative stress in CFA-induced rats which was further supported by radiological histological examination. In addition, there was significant (p < 0.05) reduction observed in pro-inflammatory cytokines, inflammatory mediators and up-regulation of anti-inflammatory cytokines in the treated group. Verbascoside was found to exhibit better biding affinities -10.4, -7.4, -7 and -6.2 kcal/mol against COX-2, IL-1β, TNF-α, and IL-6 respectively, confirmed through in silico study.
The observed outcome suggests that ETSL at a dosage of 500 mg/kg demonstrated notable anti-arthritic effects by suppressing pro-inflammatory cytokines and oxidative stress biomarkers. This effect could potentially be attributed to the presence of bioactive verbascoside identified in the LC-MS analysis.
ETHNOPHARMACOLOGICAL 相关性：Tecoma stans（L.）Juss.ex Kunth（Bignoniaceae）主要分布在非洲和亚洲的热带和亚热带地区。其叶子、花、根和树皮被用于治疗各种疾病，包括皮肤感染、肾脏问题、肠道疾病、黄疸、牙痛、关节痛和修复骨折、蛇、蝎子和老鼠咬伤的解毒剂。
本研究的目的是评估使用完全弗氏佐剂（CFA）诱导的大鼠的 Tecoma stans 叶的抗关节炎特性。
Tecoma stans 叶的乙醇提取物（ETSL）进行气相色谱-质谱联用（GC-MS）和液相色谱-质谱联用（LC-MS）分析，以鉴定潜在的生物活性物质。通过向右后爪足底表面注射 CFA（0.1ml）来进行抗关节炎活性。实验动物每天口服给予吲哚美辛（10mg/kg）和 ETSL（250、500mg/kg）一次，共 14 天。使用标准试剂盒试剂评估关节炎参数和血液学和血液生化参数。测量血清中促炎细胞因子和炎症介质的水平。评估肝匀浆和关节组织中的抗氧化参数。对关节进行放射学和组织病理学分析。对植物成分进行计算分子对接研究，以对抗 COX-2、IL-1β、IL-6 和 TNF-α 受体。
在 CFA 诱导的大鼠中，ETSL 以 500mg/kg 的剂量显著（p<0.01）恢复关节炎参数、血液学和血液生化指标以及氧化应激，这进一步得到放射学和组织病理学检查的支持。此外，在治疗组中观察到促炎细胞因子、炎症介质的显著减少（p<0.05）和抗炎细胞因子的上调。通过计算机模拟研究发现，毛蕊花糖苷表现出更好的结合亲和力-10.4、-7.4、-7 和-6.2kcal/mol，分别针对 COX-2、IL-1β、TNF-α 和 IL-6。
观察到的结果表明，ETSL 以 500mg/kg 的剂量表现出显著的抗关节炎作用，通过抑制促炎细胞因子和氧化应激生物标志物。这种作用可能归因于在 LC-MS 分析中鉴定出的生物活性毛蕊花糖苷。
