Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education & Research (DU), Porur, Chennai, India.
Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education & Research (DU), Porur, Chennai, India.
J Ethnopharmacol. 2020 Mar 1;249:112394. doi: 10.1016/j.jep.2019.112394. Epub 2019 Nov 14.
The plants selected for this study, Stereospermum colais and Stereospermum suaveolens are named as Patala in Ayurvedic medicine. Patala is a component of the reputed dasamula (ten roots) used for various imperative Ayurvedic formulations. The roots of Patala have rich traditional value especially in the treatment of inflammation and rheumatism. Nevertheless no methodical study has been consummated in these plants. Consequently, an endeavor to ascertain the anti-arthritic potential of the two plants was made besides to impart the fact with regards to the supreme one.
The aim of the study is to evaluate the anti-arthritic potential of Stereospermum colais and Stereospermum suaveolens using Complete Freund's Adjuvant induced arthritic model.
The freshly collected root material of Stereospermum colais (SC) and Stereospermum suaveolens (SS) were successively extracted with solvents such as petroleum ether, chloroform, ethyl acetate and ethanol by cold maceration and the ethanolic extract of Stereospermum colais (EESC) and Stereospermum suaveolens (EESS) were standardized using lapachol as standard. The extracts were further subjected to acute oral toxicity and in vivo anti-arthritic evaluation by Complete Freund's Adjuvant induced arthritic model using methotrexate as standard. Body weight changes, reduction in paw volume, biochemical, histopathological and the expression of Cluster of differentiation 4 cells, inflammatory cytokines such as Tumor necrosis factor-α, Interleukin-2 and Tranforming growth factor-β through immunohistochemical analysis were studied to access the anti-arthritic potential.
s: The Extracts EESC and EESC were standardized using lapachol by HPLC method and the amount was found to 3.9% w/w and 0.9% w/w respectively. The LD of the tested extracts EESC and EESS were found to be greater than 2000 mg/kg b.w through acute oral toxicity study. The extracts EESC (58.97%) and EESS (20.51%) were significantly reduced the paw volume in a dose dependent manner. The reduction in the paw volume exhibited the anti-inflammatory potential of the ethanolic extract of both the plants. The extracts at the dose of 400 mg/kg has markedly inhibited the change in joint architecture as compared to arthritic control. Also the study revealed that the extracts may possibly act by affecting the T cell mediated inflammatory process which was evident by decreased expression of Cluster of Differentiation 4 cells, Interleukin-2 and Tumor necrosis factor-α. The Extracts were found to have rich phytochemicals such as terpenoids, flavonoids, quinones, phenols and tannins may probably attribute to the anti-arthritic property of the plants. The lapachol a naphthaquinone present in both the extracts also contributed for its property. The study concludes that ethanolic extract of both the plants (EESC and EESS) exhibited significant anti-arthritic activity and Stereospermum colais was found to be more potent than Stereospermum suaveolens which corroborates the traditional use of roots of Stereospermum colais and Stereospermum suaveolens in the treatment of arthritis.
本研究中选择的植物 Stereospermum colais 和 Stereospermum suaveolens 在印度阿育吠陀医学中被称为 Patala。Patala 是著名的 dasamula（十种根）的成分之一，用于各种有影响力的阿育吠陀配方。Patala 的根具有丰富的传统价值，特别是在治疗炎症和风湿病方面。然而，这些植物尚未进行系统的研究。因此，除了了解这方面的情况外，我们还努力确定这两种植物的抗关节炎潜力。
本研究的目的是使用完全弗氏佐剂诱导的关节炎模型评估 Stereospermum colais 和 Stereospermum suaveolens 的抗关节炎潜力。
新鲜采集的 Stereospermum colais（SC）和 Stereospermum suaveolens（SS）根材料分别用石油醚、氯仿、乙酸乙酯和乙醇等溶剂通过冷浸提取，Stereospermum colais（EESC）和 Stereospermum suaveolens（EESS）的乙醇提取物用拉帕醇作为标准进行标准化。提取物进一步通过完全弗氏佐剂诱导的关节炎模型进行急性口服毒性和体内抗关节炎评价，以甲氨蝶呤作为标准。通过研究体重变化、爪体积减少、生化、组织病理学以及通过免疫组织化学分析表达的 CD4 细胞、肿瘤坏死因子-α、白细胞介素-2 和转化生长因子-β等炎症细胞因子，评估抗关节炎潜力。
结果：通过高效液相色谱法（HPLC），使用拉帕醇对提取物 EESC 和 EESC 进行了标准化，其含量分别为 3.9%w/w 和 0.9%w/w。通过急性口服毒性研究发现，测试提取物 EESC 和 EESS 的 LD 大于 2000mg/kg b.w。提取物 EESC（58.97%）和 EESS（20.51%）以剂量依赖的方式显著减少爪体积。爪体积的减少显示出两种植物的乙醇提取物的抗炎潜力。与关节炎对照组相比，400mg/kg 剂量的提取物明显抑制了关节结构的变化。此外，研究还表明，提取物可能通过影响 T 细胞介导的炎症过程发挥作用，这一点从 CD4 细胞、白细胞介素-2 和肿瘤坏死因子-α的表达减少中得到证实。提取物中含有丰富的植物化学物质，如萜类、类黄酮、醌类、酚类和单宁类，这可能是植物具有抗关节炎特性的原因。两种提取物中存在的萘醌拉帕醇也可能对其特性做出了贡献。研究结论：两种植物（EESC 和 EESS）的乙醇提取物均表现出显著的抗关节炎活性，Stereospermum colais 比 Stereospermum suaveolens 更有效，这证实了 Stereospermum colais 和 Stereospermum suaveolens 的根在治疗关节炎方面的传统用途。
