Department of Gastroenterology, Nanjing First Hospital, Nanjing Medical University, 68 Changle Road, Nanjing, Jiangsu, 210006, China.
BMC Microbiol. 2024 Oct 18;24(1):416. doi: 10.1186/s12866-024-03584-y.
Eradicating Helicobacter pylori (H. pylori) can cause an imbalance in the microbiota. Dysbiosis of the gut microbiome may produce multiple diseases and bacterial infections. The objective of this study was to investigate the influence of Helicobacter pylori (H. pylori) infection and its eradication on the composition of the oral tongue coating microbiota. A cohort of 35 participants was recruited and categorized into two groups: the H. pylori negative group (N group) consisting of 12 individuals and the H. pylori positive group (23 individuals). Within the H. pylori positive group, subjects were further stratified into the H. pylori pre-eradicated group (HPQ group) and the H. pylori eradicated group (HPH group). H. pylori positive individuals were treated with a quadruple regimen containing bismuth, and tongue coating samples were collected both prior to and following treatment. Concurrently, tongue coating samples were collected from H. pylori negative individuals. High-throughput 16S rRNA sequencing technology was employed to assess the microbial composition of the tongue coating in the N group, HPQ group, and HPH group. Pertinent clinical data were documented.Microbial diversity was found to significantly differ among the N group, HPQ group, and HPH group, as evidenced by variations in Chao1 index, Shannon index, and Partial Least Squares Discriminant Analysis (PLS-DA). The dominant bacterial phyla identified across all groups included Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, Actinobacteria, and Saccharibacteria. At the phylum level, Firmicutes exhibited higher relative abundance in the HPQ group in comparison to both the N group and HPH group. Conversely, Bacteroidetes displayed greater prevalence in the N group and HPH group. Linear Discriminant Analysis Effect Size (LEfSe) analysis indicated a higher abundance of Romboutsia, Rothia, and Turiciactor in the HPQ group. Our study revealed significant disparities in microbial diversity and richness among the three groups. Furthermore, our findings suggest a potential association between the presence of Streptococcus, Rothia and H. pylori positive individuals.
根除幽门螺杆菌（H. pylori）可能导致微生物群落失衡。肠道微生物群落的失调可能会产生多种疾病和细菌感染。本研究的目的是研究幽门螺杆菌（H. pylori）感染及其根除对口腔舌苔微生物群落组成的影响。招募了 35 名参与者，并将其分为两组：幽门螺杆菌阴性组（N 组），包括 12 人；幽门螺杆菌阳性组（23 人）。在幽门螺杆菌阳性组中，受试者进一步分为幽门螺杆菌预根除组（HPQ 组）和幽门螺杆菌根除组（HPH 组）。幽门螺杆菌阳性个体接受了包含铋的四联疗法治疗，并在治疗前后采集了舌苔样本。同时，从幽门螺杆菌阴性个体中采集了舌苔样本。采用高通量 16S rRNA 测序技术评估 N 组、HPQ 组和 HPH 组的舌苔微生物组成，并记录相关临床数据。
结果表明，N 组、HPQ 组和 HPH 组的微生物多样性存在显著差异，Chao1 指数、Shannon 指数和偏最小二乘判别分析（PLS-DA）均有所不同。所有组中鉴定的主要细菌门包括拟杆菌门、变形菌门、厚壁菌门、梭杆菌门、放线菌门和糖细菌门。在门水平上，HPQ 组的 Firmicutes 相对丰度高于 N 组和 HPH 组。相反，Bacteroidetes 在 N 组和 HPH 组中更为普遍。线性判别分析效应量（LEfSe）分析表明，HPQ 组中 Romboutsia、Rothia 和 Turiciactor 的丰度更高。
本研究表明，三组之间的微生物多样性和丰富度存在显著差异。此外，我们的研究结果表明，链球菌、Rothia 和 H. pylori 阳性个体之间可能存在关联。
