Donoso L A, Shields J A, Augsburger J J, Whitman J, Arbizo V
Arch Ophthalmol. 1986 Jan;104(1):106-10. doi: 10.1001/archopht.1986.01050130120034.
Monoclonal antibody (MAb) MAb8-1H, which identifies an ocular melanoma-associated antigen, and computer-assisted cytomorphometric analysis were used to investigate antigenic and cellular heterogeneity in four atypical cases of choroidal or ciliary body melanoma. The presence of one or more well-localized, morphologically distinct areas within each uveal melanoma, suggestive of multiple tumor cell populations, was observed by conventional light microscopy. In one choroidal melanoma with an unusual bilobed configuration, MAb8-1H bound only to the anterior lobe composed of spindle melanoma cells. In the other three cases, MAb8-1H bound selectively to discrete regions within the uveal melanoma corresponding to the well-localized, morphologically distinct areas seen by light microscopy. In each case, a computer was used to calculate the nuclear area of 50 tumor cells corresponding to the areas binding or not binding MAb8-1H. In three of the four cases examined, the differences in mean nuclear area between the discrete populations of tumor cells were statistically significant. Our results suggest that some uveal melanomas are composed of two or more discrete populations of tumor cells with regard to both expression of melanoma-associated antigens and cytomorphologic features.
识别一种眼黑色素瘤相关抗原的单克隆抗体（MAb）MAb8 - 1H以及计算机辅助细胞形态计量分析，被用于研究4例脉络膜或睫状体黑色素瘤非典型病例中的抗原性和细胞异质性。通过传统光学显微镜观察到，每例葡萄膜黑色素瘤内存在一个或多个定位良好、形态学上不同的区域，提示存在多个肿瘤细胞群体。在1例具有不寻常双叶结构的脉络膜黑色素瘤中，MAb8 - 1H仅与由梭形黑色素瘤细胞组成的前叶结合。在其他3例病例中，MAb8 - 1H选择性地与葡萄膜黑色素瘤内对应于光学显微镜下所见定位良好、形态学上不同区域的离散区域结合。在每例病例中，使用计算机计算对应于结合或未结合MAb8 - 1H区域的50个肿瘤细胞的核面积。在所检查的4例病例中的3例，肿瘤细胞离散群体之间的平均核面积差异具有统计学意义。我们的结果表明，就黑色素瘤相关抗原的表达和细胞形态学特征而言，一些葡萄膜黑色素瘤由两个或更多离散的肿瘤细胞群体组成。
