Cobbett P, Smithson K G, Hatton G I
Brain Res. 1986 Jan 1;362(1):7-16. doi: 10.1016/0006-8993(86)91392-2.
Bursts of action potentials were recorded intracellularly from 11 phasically firing magnocellular neurons in the paraventricular nucleus in slices of rat hypothalamus. The bursts of overshooting, often broadening action potentials (63-87 mV peak-to-peak) were superimposed on depolarizing plateau potentials. Phasic activity was recorded before and/or after the neurons were injected with the fluorescent dye Lucifer Yellow CH. Injected neurons were first examined in whole slices, and subsequently, in sectioned material, characterized immunocytochemically using antisera to vasopressin- and oxytocin-associated neurophysins (VP-NP and OT-NP respectively). The 11 injections produced 8 single dye filled neurons and 3 pairs of dye-coupled neurons, 14 dye-filled cells in all. Six of the single cells and all the dye coupled pairs were immunoreactive with VP-NP antiserum and not reactive with OT-NP antiserum. Most of these neurons were in areas of the nucleus in which VP-NP reactive cells predominated, but two were surrounded by OT-NP reactive cells. Two single, dye-filled, phasically active, magnocellular neurons failed to show immunoreactivity to either antiserum.
在大鼠下丘脑切片的室旁核中，对11个呈相位性放电的大细胞神经元进行了细胞内动作电位记录。超射性动作电位爆发，通常动作电位变宽（峰峰值63 - 87 mV），叠加在去极化平台电位上。在向神经元注射荧光染料路西法黄CH之前和/或之后记录相位活动。首先在完整切片中检查注射后的神经元，随后在切片材料中，使用抗血管加压素和催产素相关神经垂体素（分别为VP - NP和OT - NP）的抗血清进行免疫细胞化学鉴定。11次注射产生了8个单染神经元和3对染料耦联神经元，共14个染有染料的细胞。6个单细胞和所有染料耦联对与VP - NP抗血清呈免疫反应，而与OT - NP抗血清无反应。这些神经元大多数位于核内VP - NP反应性细胞占主导的区域，但有两个被OT - NP反应性细胞包围。两个单染、有相位活动的大细胞神经元对两种抗血清均未表现出免疫反应性。
