Falev Danil I, Onuchina Alexandra A, Faleva Anna V, Voronov Ilya S, Kosyakov Dmitry S, Ulyanovskii Nikolay V
Laboratory of Natural Compounds Chemistry and Bioanalytics, Core Facility Center "Arktika", M.V. Lomonosov Northern (Arctic) Federal University, Arkhangelsk, Russia.
Nat Prod Res. 2024 Oct 20:1-8. doi: 10.1080/14786419.2024.2417837.
Cloudberry (.) is a plant rich in various biologically active compounds. In this work, the composition and concentrations of pentacyclic triterpenoids (PCTs) and phytosterols in various parts of cloudberries were studied using high-performance liquid chromatography-tandem mass spectrometry by multiple reaction monitoring mode (MRM, targeted analysis) and precursor ion scan mode (non-targeted analysis and semi-quantitative determination). Moderately polar fractions of cloudberry leaves, sepals and berries extracts are rich in β-sitosterol glycoside and various triterpenoid acids, among which tormentic acid (∼190-1800 μg/g dw part of cloudberries) and its hydroxyl derivatives (∼3.1-470 μg/g dw part of cloudberries) predominate. Non-polar fractions are characterised by the presence of amyrins (0.76-110 μg/g dw part of cloudberries), β-sitosterol (62-750 µg/g dw part of cloudberries) and β-sitosterol glycoside (∼92-360 μg/g dw sepals).
云莓（.）是一种富含多种生物活性化合物的植物。在这项工作中，采用高效液相色谱 - 串联质谱法，通过多反应监测模式（MRM，靶向分析）和前体离子扫描模式（非靶向分析和半定量测定）研究了云莓各部位五环三萜（PCTs）和植物甾醇的组成及含量。云莓叶、萼片和浆果提取物的中等极性部分富含β - 谷甾醇糖苷和各种三萜酸，其中 tormentic 酸（约190 - 1800μg/g 云莓干重部分）及其羟基衍生物（约3.1 - 470μg/g 云莓干重部分）占主导。非极性部分的特征是存在羽扇豆醇（0.76 - 110μg/g 云莓干重部分）、β - 谷甾醇（62 - 750μg/g 云莓干重部分）和β - 谷甾醇糖苷（约92 - 360μg/g 萼片干重）。
