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C-X-C基序趋化因子配体12在与化疗耐药、放疗抵抗以及干性相关的结直肠癌中的作用

The Effect of C-X-C Motif Chemokine Ligand 12 in Colorectal Cancer Associated with Chemoresistance and Radioresistance as Well as Stemness.

作者信息

Dong Wuzhen, Lin Wen, Li Chong

机构信息

Department of Proctology, Jinhua Central Hospital, Jinhua, Zhejiang, China.

Department of Nursing, Ziyang College of Dental Technology, Ziyang, Sichuan, China.

出版信息

Iran J Public Health. 2024 Sep;53(9):2079-2089. doi: 10.18502/ijph.v53i9.16461.

DOI:10.18502/ijph.v53i9.16461
PMID:39429654
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11490330/
Abstract

BACKGROUND

We aimed to explore the role of C-X-C motif chemokine ligand 12 ( and cytokinecytokine receptor interaction signaling pathway in the radiotherapy and chemotherapy resistance as well as cell stemness in colorectal cancer (CRC).

METHODS

Bioinformatics analysis was used to identify the differentially expressed mRNAs and signal pathways closely related to differentially expressed mRNAs have also been analyzed in March 2022 at the Jinhua Central Hospital, China. Then, the expression of was detected by qRT-PCR in colorectal cancer cells and testing the effects of transfecting into different CRC-derived cell lines. The effects of on cell proliferation were evaluated by chemosensitivity assay and radiation sensitivity assay.

RESULTS

Bioinformatics analysis of DEGs found a total of 2429 differentially expressed genes, and genes are two abnormally highly expressed genes in the CRC. KEGG analysis showed the correlative signaling pathway, cytokine-cytokine receptor interaction, which is related to cell stemness. Furthermore, the expression of in CRC cells was detected and an increasing trend was obtained in CRC cells. In addition, the chemosensitivity and radiotherapy tolerance were elevated after transfected with .

CONCLUSION

CXCL12 could be a potential promote biomarkers in CRC and also promote the chemosensitivity and radiotherapy tolerance.

摘要

背景

我们旨在探讨C-X-C基序趋化因子配体12（CXCL12）及细胞因子-细胞因子受体相互作用信号通路在结直肠癌（CRC）放疗和化疗耐药以及细胞干性中的作用。

方法

采用生物信息学分析来鉴定差异表达的mRNA，并对与差异表达mRNA密切相关的信号通路进行分析。2022年3月在中国金华市中心医院也进行了相关分析。然后，通过qRT-PCR检测结直肠癌细胞中CXCL12的表达，并测试将其转染到不同CRC来源细胞系中的效果。通过化学敏感性试验和放射敏感性试验评估CXCL12对细胞增殖的影响。

结果

对差异表达基因（DEGs）的生物信息学分析共发现2429个差异表达基因，CXCL12和[未明确基因名称]基因是CRC中两个异常高表达的基因。KEGG分析显示相关信号通路为细胞因子-细胞因子受体相互作用，这与细胞干性有关。此外，检测了CRC细胞中CXCL12的表达，并发现其在CRC细胞中有上升趋势。另外，转染CXCL12后化学敏感性和放疗耐受性升高。

结论

CXCL12可能是CRC中一种潜在的促进生物标志物，并且还能促进化学敏感性和放疗耐受性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/495c/11490330/d07cd8daa07f/IJPH-53-2079-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/495c/11490330/f612ac6fac7a/IJPH-53-2079-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/495c/11490330/d07cd8daa07f/IJPH-53-2079-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/495c/11490330/f612ac6fac7a/IJPH-53-2079-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/495c/11490330/99bfdb264b39/IJPH-53-2079-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/495c/11490330/d07cd8daa07f/IJPH-53-2079-g007.jpg

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