The Effect of C-X-C Motif Chemokine Ligand 12 in Colorectal Cancer Associated with Chemoresistance and Radioresistance as Well as Stemness.

BACKGROUND

We aimed to explore the role of C-X-C motif chemokine ligand 12 ( and cytokinecytokine receptor interaction signaling pathway in the radiotherapy and chemotherapy resistance as well as cell stemness in colorectal cancer (CRC).

METHODS

Bioinformatics analysis was used to identify the differentially expressed mRNAs and signal pathways closely related to differentially expressed mRNAs have also been analyzed in March 2022 at the Jinhua Central Hospital, China. Then, the expression of was detected by qRT-PCR in colorectal cancer cells and testing the effects of transfecting into different CRC-derived cell lines. The effects of on cell proliferation were evaluated by chemosensitivity assay and radiation sensitivity assay.

RESULTS

Bioinformatics analysis of DEGs found a total of 2429 differentially expressed genes, and genes are two abnormally highly expressed genes in the CRC. KEGG analysis showed the correlative signaling pathway, cytokine-cytokine receptor interaction, which is related to cell stemness. Furthermore, the expression of in CRC cells was detected and an increasing trend was obtained in CRC cells. In addition, the chemosensitivity and radiotherapy tolerance were elevated after transfected with .

CONCLUSION

CXCL12 could be a potential promote biomarkers in CRC and also promote the chemosensitivity and radiotherapy tolerance.