Bolognesi C, Boffa L C
Cancer Lett. 1986 Jan;30(1):91-5. doi: 10.1016/0304-3835(86)90137-0.
Mice from strains with different susceptibility to the colon-specific carcinogen 1,2-dimethylhydrazine (DMH) were tested for DNA damage in liver, kidney and colon after administration of the compound at a dosage that has been reported to induce a high incidence of adenocarcinoma in the colon of rodents. DNA breaks were evaluated from their elution rate constant according to the alkaline elution technique. We found that 4 h after administration of the carcinogen there was a substantial and comparable DNA damage in liver and kidney of all strains examined. Conversely, colon DNA damage was hardly above control levels in the carcinogen-resistant strains. The highest DNA damage was detected in the most susceptible strain and was slightly lower in the two other susceptible strains. We propose that the extent of DNA breakage in a target organ could be one of the factors determining organ-specific and strain-specific susceptibility to DMH.
选用对结肠特异性致癌物1,2 - 二甲基肼（DMH）具有不同易感性的品系小鼠，在给予已报道可在啮齿动物结肠诱发高腺癌发生率剂量的该化合物后，检测其肝脏、肾脏和结肠中的DNA损伤。根据碱性洗脱技术，从其洗脱速率常数评估DNA断裂情况。我们发现，给予致癌物4小时后，所有检测品系的肝脏和肾脏中均出现了大量且相当的DNA损伤。相反，在致癌物抗性品系中，结肠DNA损伤几乎未超过对照水平。在最易感品系中检测到的DNA损伤最高，另外两个易感品系中的损伤略低。我们提出，靶器官中DNA断裂的程度可能是决定对DMH的器官特异性和品系特异性易感性的因素之一。
